Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002032
UniProt IDQ9UKX7
Primary gene name(s)NUP50
Synonym gene name(s)NPAP60L
Protein nameNuclear pore complex protein Nup50
Protein functionComponent of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. {ECO:0000269|PubMed:20016008}.
Subcellular locationNucleus, nuclear pore complex {ECO:0000269|PubMed:12802065}. Nucleus membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250};
Nucleoplasmic side {ECO:0000250}. Note=Localizes to the nucleoplasmic fibrils of the nuclear pore complex, By similarity. Dissociates from the NPC structure early during prophase of mitosis. Associates to the newly formed nuclear membrane during telophase. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UKX7
Gene Ontology
(Biological Process)
Complete annatation
gene silencing by RNA [GO:0031047];
intracellular transport of virus [GO:0075733];
mitotic nuclear envelope disassembly [GO:0007077];
mRNA export from nucleus [GO:0006406];
protein import into nucleus [GO:0006606];
protein sumoylation [GO:0016925];
regulation of cellular response to heat [GO:1900034];
regulation of glucose transport [GO:0010827];
tRNA export from nucleus [GO:0006409];
viral process [GO:0016032];
viral transcription [GO:0019083]
Gene Ontology
(Molecular Function)
Complete annatation
Ran GTPase binding [GO:0008536]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nuclear membrane [GO:0031965];
nuclear pore [GO:0005643];
nucleoplasm [GO:0005654]
Protein-protein interaction115982
Phylogenetic treeQ9UKX7
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.356393579773910.276674367576490.392178695392914
AZA vs. DISU-0.2105731022252110.4046321481940890.905959666736916
AZA vs. IL70.1585577491482760.4088173810047280.999311006273513
AZA vs. SAHA-0.2801165205294840.2505458565047230.620356149959295
DISU vs. CD3-0.5790065168899070.1111699102302410.202559558420241
DISU vs. IL70.3602007813707020.1527921605872120.507840368746006
DISU vs. SAHA-0.06888627015925530.8128653374763780.95143627687997
DMSO vs. AZA-0.04296547687435470.7971754520125511
DMSO vs. CD3-0.4108240775761130.199277777813480.298266753271768
DMSO vs. DISU0.1657081687205190.4964972035780050.907374831181244
DMSO vs. IL70.2088030534663720.2448880486694370.748610000584892
DMSO vs. SAHA-0.2443901360653060.2994961300869440.652201695915001
HIV vs. Mock in Activation-0.0009550898225390560.9987753054834690.999983755607037
HIV vs. Mock in Latency-0.09080885368379610.5816572413551080.999834320637052
IL7 vs. CD3-0.189467633370160.5548007757241980.677606934714786
SAHA vs. CD3-0.6618241242385940.06208609876872240.118660432269454
SAHA vs. IL7-0.442432573016650.0695024943941960.229001224152592
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.106907 0.510288
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.983 0.944 0.917 0.926 0.978
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2EC1 NMR - A=351-468.
3TJ3 X-ray 2.7Å C/D=1-109.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)
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