Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002030
UniProt IDQ8NFH5
Primary gene name(s)NUP35
Synonym gene name(s)MP44, NUP53
Protein nameNucleoporin NUP53
Protein functionFunctions as a component of the nuclear pore complex, NPC. NPC components, collectively referred to as nucleoporins, NUPs. Can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC. {ECO:0000269|PubMed:15703211}.
Subcellular locationNucleus, nuclear pore complex {ECO:0000269|PubMed:12196509}. Nucleus membrane {ECO:0000269|PubMed:15703211};
Peripheral membrane protein {ECO:0000269|PubMed:15703211}. Note=Tightly associated with the nuclear membrane and lamina. {ECO:0000269|PubMed:15703211}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8NFH5
Gene Ontology
(Biological Process)
Complete annatation
gene silencing by RNA [GO:0031047];
intracellular transport of virus [GO:0075733];
mitotic nuclear envelope disassembly [GO:0007077];
mRNA export from nucleus [GO:0006406];
NLS-bearing protein import into nucleus [GO:0006607];
nuclear pore organization [GO:0006999];
protein sumoylation [GO:0016925];
regulation of cellular response to heat [GO:1900034];
regulation of glucose transport [GO:0010827];
regulation of transcription, DNA-templated [GO:0006355];
transmembrane transport [GO:0055085];
tRNA export from nucleus [GO:0006409];
viral process [GO:0016032];
viral transcription [GO:0019083]
Gene Ontology
(Molecular Function)
Complete annatation
nucleocytoplasmic transporter activity [GO:0005487];
nucleotide binding [GO:0000166];
phospholipid binding [GO:0005543];
single-stranded DNA binding [GO:0003697]
Gene Ontology
(Cellular Component)
Complete annatation
intermediate filament cytoskeleton [GO:0045111];
intracellular membrane-bounded organelle [GO:0043231];
nuclear envelope [GO:0005635];
nuclear lamina [GO:0005652];
nuclear membrane [GO:0031965];
nuclear pore central transport channel [GO:0044613];
nuclear pore nuclear basket [GO:0044615];
nucleoplasm [GO:0005654];
plasma membrane [GO:0005886]
Protein-protein interaction126190
Phylogenetic treeQ8NFH5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.23869311960687.76869679697256e-111.38567808430734e-09
AZA vs. DISU0.09973806825456230.699611780361530.969609310121983
AZA vs. IL70.4639982426899030.0200759242582470.458244372746071
AZA vs. SAHA-0.4787402257786130.05827696581571170.29628636506288
DISU vs. CD3-2.151381339676631.37252491594353e-082.04643329208039e-07
DISU vs. IL70.3553772976823060.1658726519413570.52820663878664
DISU vs. SAHA-0.577601849421640.05277867612605210.288093961570348
DMSO vs. AZA-0.03535713625594390.8420374661088161
DMSO vs. CD3-2.286254917233441.12491127524095e-112.07173451852138e-10
DMSO vs. DISU-0.1371211593505870.5824007277169240.932784987235767
DMSO vs. IL70.5064393474797570.006767956393279760.171762529677933
DMSO vs. SAHA-0.4496641702657180.0656935361287790.296693821695184
HIV vs. Mock in Activation-0.08724537314268720.8885632245023830.999983755607037
HIV vs. Mock in Latency-0.05590943839894740.7489325200492570.999834320637052
IL7 vs. CD3-1.766436488533321.03122359762509e-071.25150026465402e-06
SAHA vs. CD3-2.742561772018974.11559675228546e-131.33094512997729e-11
SAHA vs. IL7-0.9460814007484350.0001746767356514130.00301612498649416
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.513358 0.00108419
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.949 0.851 0.674 0.581 0.809
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4LIR X-ray 2.4Å A/B=151-266.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)