Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002029
UniProt IDP35658
Primary gene name(s)NUP214
Synonym gene name(s)CAIN, CAN, KIAA0023
Protein nameNuclear pore complex protein Nup214
Protein functionMay serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.
Subcellular locationNucleus, nuclear pore complex. Note=Cytoplasmic filaments.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P35658
Gene Ontology
(Biological Process)
Complete annatation
gene silencing by RNA [GO:0031047];
intracellular transport of virus [GO:0075733];
mitotic nuclear envelope disassembly [GO:0007077];
mRNA export from nucleus [GO:0006406];
protein export from nucleus [GO:0006611];
protein import into nucleus [GO:0006606];
protein sumoylation [GO:0016925];
regulation of cell cycle [GO:0051726];
regulation of cellular response to heat [GO:1900034];
regulation of glucose transport [GO:0010827];
regulation of mRNA stability [GO:0043488];
RNA export from nucleus [GO:0006405];
tRNA export from nucleus [GO:0006409];
viral process [GO:0016032];
viral transcription [GO:0019083]
Gene Ontology
(Molecular Function)
Complete annatation
nuclear export signal receptor activity [GO:0005049];
transporter activity [GO:0005215]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
focal adhesion [GO:0005925];
intracellular membrane-bounded organelle [GO:0043231];
nuclear pore [GO:0005643];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction113717
Phylogenetic treeP35658
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3814756531796490.2445990362309910.356124099118382
AZA vs. DISU0.1725896367997050.4957094040593190.934608728211275
AZA vs. IL70.06415159978229640.7392143533217180.999311006273513
AZA vs. SAHA-0.1784404303290550.4651982170791250.802517501058679
DISU vs. CD3-0.2214510964452970.5422427760375380.662272533300101
DISU vs. IL7-0.1178323365270040.6400553757248060.89399006662202
DISU vs. SAHA-0.3488662060556770.2318934426025290.609334351976746
DMSO vs. AZA0.07868467500228870.6396617658063711
DMSO vs. CD3-0.3144099377838410.3262094206150970.439581274977377
DMSO vs. DISU-0.09588396970129590.6944819374656110.958674191480009
DMSO vs. IL7-0.007048339458806680.9687891898184830.993893941764584
DMSO vs. SAHA-0.2627749178616590.2657462733476310.614495694481925
HIV vs. Mock in Activation0.2973969792545480.6331596843430250.999983755607037
HIV vs. Mock in Latency0.07731762083602680.6404105963201580.999834320637052
IL7 vs. CD3-0.3113195942161660.3336309438188790.472056624066645
SAHA vs. CD3-0.5838025962446060.09969340218624330.173531042550575
SAHA vs. IL7-0.2454462712663690.3142275551542970.563198008001014
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0662005 0.741784
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.012 0.92 0.93 0.974 0.952
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2OIT X-ray 1.6Å A=1-434.
3FHC X-ray 2.8Å A=1-405.
3FMO X-ray 2.5Å A=1-450.
3FMP X-ray 3.1Å A/C=1-450.
5DIS X-ray 2.8Å D=1916-2027.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 11238447
Rev recruits 9847314
Rev inhibited by 9765402
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)