Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002024
UniProt IDP49790
Primary gene name(s)NUP153
Synonym gene name(s)unknown
Protein nameNuclear pore complex protein Nup153
Protein functionComponent of the nuclear pore complex, NPC, a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element, CTE in a NXF1- and KHDRBS1-independent manner. Mediates TPR anchoring to the nuclear membrane at NPC. The repeat-containing domain may be involved in anchoring other components of the NPC to the pore membrane. Possible DNA-binding subunit of the nuclear pore complex, NPC. {ECO:0000269|PubMed:12802065, ECO:0000269|PubMed:15229283, ECO:0000269|PubMed:22253824}.
Subcellular locationNucleus. Nucleus membrane. Nucleus, nuclear pore complex. Note=Tightly associated with the nuclear membrane and lamina, By similarity. Localized to the nucleoplasmic side of the nuclear pore complex, NPC core structure, forming a fibrous structure called the nuclear basket. Dissociates from the NPC structure early during prophase of mitosis. Integrated in the newly assembled nuclear envelope of postmitotic cells early in G1. Colocalized with NUP98 and TPR to the nuclear basket at the nucleoplasmic side of the NPC. Detected in diffuse and discrete intranuclear foci. Remained localized to the nuclear membrane after poliovirus, PV infection. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P49790
Gene Ontology
(Biological Process)
Complete annatation
gene silencing by RNA [GO:0031047];
intracellular transport of virus [GO:0075733];
mitotic nuclear envelope disassembly [GO:0007077];
mRNA export from nucleus [GO:0006406];
negative regulation of RNA export from nucleus [GO:0046832];
nuclear pore complex assembly [GO:0051292];
protein import into nucleus [GO:0006606];
protein sumoylation [GO:0016925];
regulation of cellular response to heat [GO:1900034];
regulation of glucose transport [GO:0010827];
tRNA export from nucleus [GO:0006409];
viral entry into host cell [GO:0046718];
viral penetration into host nucleus [GO:0075732];
viral process [GO:0016032];
viral transcription [GO:0019083]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
double-stranded DNA binding [GO:0003690];
identical protein binding [GO:0042802];
nuclear localization sequence binding [GO:0008139];
nucleocytoplasmic transporter activity [GO:0005487];
protein anchor [GO:0043495];
structural constituent of nuclear pore [GO:0017056];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
annulate lamellae [GO:0005642];
cytoplasm [GO:0005737];
nuclear inclusion body [GO:0042405];
nuclear membrane [GO:0031965];
nuclear periphery [GO:0034399];
nuclear pore [GO:0005643];
nuclear pore nuclear basket [GO:0044615];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654]
Protein-protein interaction115297
Phylogenetic treeP49790
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      Yes
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.108790313936460.0008038207485207010.00299732467137655
AZA vs. DISU0.3569517194179410.1586912368146750.740591858744402
AZA vs. IL70.2024568754136150.2922949022023160.999311006273513
AZA vs. SAHA0.1101223976232330.6516223177519650.893609426655124
DISU vs. CD3-0.765138828241810.03568196943541160.0823531087967537
DISU vs. IL7-0.1633497383605690.5170803426032450.835624786176853
DISU vs. SAHA-0.2454179706907340.3993881852717540.760024414351585
DMSO vs. AZA-0.01423729408011640.9322755944202821
DMSO vs. CD3-1.133620306803170.0004532009864816060.00171372310776742
DMSO vs. DISU-0.3729344528712290.1268758105393720.612433845236493
DMSO vs. IL70.2239651721510780.2129416707945330.722716647686679
DMSO vs. SAHA0.1172177358945630.6189705449480990.875136319220589
HIV vs. Mock in Activation0.2285597098335160.7145477547574870.999983755607037
HIV vs. Mock in Latency0.04661737246571520.7774378385921810.999834320637052
IL7 vs. CD3-0.8985626973090860.005445115522982320.0180026817894083
SAHA vs. CD3-1.023763673684370.004082518382512060.0120018729373218
SAHA vs. IL7-0.09586894010184310.6939165877855020.857157409872337
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.2987 0.04032

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.261191 0.0477171
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.917 0.675 0.573 0.605 0.726
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2EBQ NMR - A=722-761.
2EBR NMR - A=851-890.
2EBV NMR - A=773-822.
2GQE NMR - A=722-750.
4U0C X-ray 1.7Å B=1407-1423.
4U0D X-ray 3.0Å M/N/O/P/Q/R=1407-1423.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr interacts with 15659641
21318276
21489275
Rev interacts with 11238447
capsid binds 23523133
2503688624130490
25356722
2551886125356722
25518861
integrase interacts with 18854154
18984154
20227665
21327100
21593146
21994675
22082286
22928108
2514721219369352
21593146
nucleocapsid binds 23523133
Rev inhibited by 10069809
integrase binds 19369352
HIV-1 virus replication enhanced by expression of human gene 18854154
18187620
2573116123523133
Tat interacts with 19454010
capsid interacts with 21593146
22082286
22174692
23883001
24051001
24525292
2503688623097450

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03013 RNA transport - Homo sapiens (human)
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