Protein Information (annotations from UniProt)
| Database ID | HIV0002022 |
| UniProt ID | P49757 |
| Primary gene name(s) | NUMB |
| Synonym gene name(s) | unknown |
| Protein name | Protein numb homolog |
| Protein function | Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells, RGCs, by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone, SVZ neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. |
| Subcellular location | Membrane; Peripheral membrane protein. |
| ECO code | Click here for more information. |
| Amino acid sequence FASTA format: P49757 |
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| Gene Ontology (Biological Process) Complete annatation | adherens junction organization [GO:0034332]; axonogenesis [GO:0007409]; lateral ventricle development [GO:0021670]; lung epithelial cell differentiation [GO:0060487]; negative regulation of Notch signaling pathway [GO:0045746]; negative regulation of protein localization to plasma membrane [GO:1903077]; neuroblast division in subventricular zone [GO:0021849]; positive regulation of cell migration [GO:0030335]; positive regulation of neurogenesis [GO:0050769]; positive regulation of polarized epithelial cell differentiation [GO:0030862] |
| Gene Ontology (Molecular Function) Complete annatation | unknown |
| Gene Ontology (Cellular Component) Complete annatation | apical part of cell [GO:0045177]; basolateral plasma membrane [GO:0016323]; clathrin-coated vesicle [GO:0030136]; early endosome [GO:0005769]; extrinsic component of plasma membrane [GO:0019897]; focal adhesion [GO:0005925]; nucleus [GO:0005634]; plasma membrane [GO:0005886] |
| Protein-protein interaction | 114202 |
| Phylogenetic tree | P49757 |
| HIV replication factor status |
Zhou et al., Cell. Host. Microbe., 2008 unknown Brass et al., Science, 2008 unknown Smith et al., J. Immunol, 2010 unknown |
| Interferon-stimulated gene status |
Lu et al., J. Virol., 2011 Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown; Schoggins JW and Rice CM, Curr. Opin. Virol., 2011 Targeted viruses: unknown Viral life cycle: unknown Mechanism related to antiviral activity: unknown |
| Anti-viral restriction factor |
Liu et al., Retrovirology, 2011 unknown (Triplicates) |
Gene Expression Profile top
Up-regulated; 
Down-regulated
For brief introduction to each study, please go to the help page.
Gene expression during HIV latency
| (1). Mohammadi et al., PLoS Pathog., 2014 Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model DMSO: Dimethyl suloxyde (negative control) - 0.0033% final SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies IL7: Interleukin-7 based stimulation DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM |
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| Experimental Condition | Log2 Fold Change | P value | Adjusted P value |
| AZA vs. CD3 | 0.0911271710483057 | 0.780759678384716 | 0.849646922210429 |
| AZA vs. DISU | 0.00777179000808363 | 0.975511122793319 | 0.997811953897277 |
| AZA vs. IL7 | -0.0714917584660211 | 0.710950583614611 | 0.999311006273513 |
| AZA vs. SAHA | 0.132694049746836 | 0.587100792488324 | 0.863157841214181 |
| DISU vs. CD3 | -0.0959237426484259 | 0.791260490972277 | 0.856217528060801 |
| DISU vs. IL7 | -0.088386565386881 | 0.725933413998956 | 0.930944114950367 |
| DISU vs. SAHA | 0.126458451005084 | 0.664661842626523 | 0.89799360199232 |
| DMSO vs. AZA | -0.0264278591981141 | 0.87518687137657 | 1 |
| DMSO vs. CD3 | -0.129114528016338 | 0.686474685061511 | 0.769074226998711 |
| DMSO vs. DISU | -0.0361094446262105 | 0.882434552704014 | 0.987443803738299 |
| DMSO vs. IL7 | -0.0377876790623214 | 0.834080215431661 | 0.963494783989913 |
| DMSO vs. SAHA | 0.152680814396972 | 0.518144389258367 | 0.822514650359474 |
| HIV vs. Mock in Activation | 0.142024489011084 | 0.819487681448518 | 0.999983755607037 |
| HIV vs. Mock in Latency | 0.030313184002114 | 0.854728663675727 | 0.999834320637052 |
| IL7 vs. CD3 | -0.155208795962049 | 0.629458893798981 | 0.741129708609797 |
| SAHA vs. CD3 | 0.0175003005098498 | 0.960469395583147 | 0.973518549329972 |
| SAHA vs. IL7 | 0.200867042199865 | 0.411083558314146 | 0.656671636810115 |
| (2). Iglesias-Ussel et al., J. Virol., 2013 Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model |
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| Log2 Fold Change | P Value | ||
| unknown | unknown | ||
Gene expression during HIV infection and replication
| (1). Imbeault et al., PloS Pathog., 2012 Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells |
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| Experiment type | Log2 Fold Change | P Value | Adjusted P Value | ||
| Infected vs. Mock | unknown | unknown | unknown | ||
| Infected vs. Bystander | unknown | unknown | unknown | ||
| (2). Lefebvre et al., J. Virol., 2011 Transcriptome analysis of T-cell line (Sup T1) |
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| Log2 Fold Change | unknown | ||||
| (3). Li et al., J. Immunol., 2013 Lymphatic tissue |
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| Acute Fold Change | Acute P Value | Asymt Fold Change | Asypt P Value | AIDS Fold Change | AIDS P Value |
| unknown | unknown | unknown | unknown | unknown | unknown |
| (4). Chang et al., MBio., 2011 Transcriptome analysis of T-cell line (Sup T1) Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation |
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| Up-regulated (True) | TRUE | ||||
| (5). Sherrill-Mix et al., BMC Retrovirol., 2015 Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based |
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| Test Status | Log2 Fold Change | P Value | |||
| OK | 0.586839 | 0.000580925 | |||
| (6). Rotger et al., PLoS Pathog., 2010 Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient (Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach) |
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| Log2 Fold Change | P Value | ||||
| unknown | unknown | ||||
Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV
| (1). Greenwood et al., Elife, 2016 Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset |
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| 6 h | 24 h | 48 h | 72 h | RTi | ||
| 1.058 | 1.009 | 1.104 | 1.193 | 0.989 | ||
| (2). Navare et al., Virology, 2012 SUP-T1 cell line |
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| FC-4hpi | P-value | FC-8hpi | P-value | FC-20hpi | P-value | Category |
| unknown | unknown | unknown | unknown | unknown | unknown | unknown |
| (3). Hyrcza et al., J. Virolo., 2007 Primary human CD4+ and CD8+ T Cells |
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| Affymetrix Prob ID | Fold Change | In CD8? | Category | |||
| unknown | unknown | unknown | unknown | |||
Protein Overview top
Drug-protein Interaction (annotations from DrugBank) top
Protein Secondary Structure (annotations from PDB) top
HIV-1 Interaction (annotations from NCBI HIV-1 Interaction Database) top
Metabolic/Signalling Pathway (annotations from KEGG database) top
| Pathway Accession Number | Description |
|---|---|
| hsa04330 | Notch signaling pathway - Homo sapiens (human) |
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