Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0002007
UniProt IDP46459
Primary gene name(s)NSF
Synonym gene name(s)unknown
Protein nameVesicle-fusing ATPase
Protein functionRequired for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling, By similarity. {ECO:0000250}.
Subcellular locationCytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P46459
Gene Ontology
(Biological Process)
Complete annatation
COPII vesicle coating [GO:0048208];
ER to Golgi vesicle-mediated transport [GO:0006888];
exocytosis [GO:0006887];
Golgi to plasma membrane protein transport [GO:0043001];
Golgi vesicle docking [GO:0048211];
intracellular protein transport [GO:0006886];
intra-Golgi vesicle-mediated transport [GO:0006891];
plasma membrane fusion [GO:0045026];
positive regulation of protein catabolic process [GO:0045732];
positive regulation of receptor recycling [GO:0001921];
potassium ion transport [GO:0006813];
regulation of exocytosis [GO:0017157];
retrograde vesicle-mediated transport, Golgi to ER [GO:0006890];
SNARE complex disassembly [GO:0035494];
vesicle-mediated transport [GO:0016192]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase activity [GO:0016887];
ATPase activity, coupled [GO:0042623];
ATP binding [GO:0005524];
metal ion binding [GO:0046872];
PDZ domain binding [GO:0030165];
protein complex binding [GO:0032403];
protein kinase binding [GO:0019901];
Rab GTPase binding [GO:0017137];
syntaxin-1 binding [GO:0017075]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
dendritic shaft [GO:0043198];
extracellular exosome [GO:0070062];
Golgi membrane [GO:0000139];
Golgi stack [GO:0005795];
lysosomal membrane [GO:0005765];
myelin sheath [GO:0043209];
plasma membrane [GO:0005886];
postsynaptic density [GO:0014069]
Protein-protein interaction110960
Phylogenetic treeP46459
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5935073464019350.07091788149229660.132680525056094
AZA vs. DISU-0.05201231656314970.8376656403867040.98640511391213
AZA vs. IL70.002990856086724620.9876604988799880.999311006273513
AZA vs. SAHA-0.0360317816260650.8829430478249540.9746045322157
DISU vs. CD3-0.6570854583818630.07104677254956030.142566433627805
DISU vs. IL70.04552791898375760.8569027486374470.972095797044404
DISU vs. SAHA0.01727160660469580.9528820958345380.989441867353937
DMSO vs. AZA-0.135016318703170.4242503179045461
DMSO vs. CD3-0.7379232631815630.02172156897609520.0479797937907564
DMSO vs. DISU-0.08437201399199050.7304523763544870.966285722215104
DMSO vs. IL70.1449648891953410.4232093664159720.854720785525968
DMSO vs. SAHA0.09204990896251680.6971593699052160.908046523297791
HIV vs. Mock in Activation0.1749045525627920.7785918115382820.999983755607037
HIV vs. Mock in Latency0.1171843473103980.4807331815356240.999834320637052
IL7 vs. CD3-0.5835192192499460.07025248922113150.143892327677248
SAHA vs. CD3-0.653733008301070.06541473325900530.123766482135996
SAHA vs. IL7-0.04186879829810710.8639924842147640.943820462042602
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.762503 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.996 1.074 1.338 1.534 1.347
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB01902 1-Ethyl-Pyrrolidine-2,5-Dione experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) inhibited by 21680744
Envelope surface glycoprotein gp120 complexes with 23125841
Envelope surface glycoprotein gp160; precursor inhibited by 21680744
Gag-Pol complexes with 23125841
Pr55(Gag) complexes with 23125841
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04721 Synaptic vesicle cycle - Homo sapiens (human)
hsa04727 GABAergic synapse - Homo sapiens (human)
hsa04962 Vasopressin-regulated water reabsorption - Homo sapiens (human)