Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001983
UniProt IDQ15233
Primary gene name(s)NONO
Synonym gene name(s)NRB54
Protein nameNon-POU domain-containing octamer-binding protein
Protein functionDNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining, NHEJ required for double-strand break repair and V(DJ recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5, Ku dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles, IAPs and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Important for the functional organization of GABAergic synapses. Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript. {ECO:0000250|UniProtKB:Q99K48, ECO:0000269|PubMed:10858305, ECO:0000269|PubMed:11525732, ECO:0000269|PubMed:11897684, ECO:0000269|PubMed:15590677, ECO:0000269|PubMed:22416126, ECO:0000269|PubMed:26571461}.
Subcellular locationNucleus. Nucleus, nucleolus. Nucleus speckle. Note=Detected in punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q15233
Gene Ontology
(Biological Process)
Complete annatation
circadian rhythm [GO:0007623];
DNA recombination [GO:0006310];
DNA repair [GO:0006281];
mRNA processing [GO:0006397];
mRNA splicing, via spliceosome [GO:0000398];
negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway [GO:1903377];
negative regulation of transcription, DNA-templated [GO:0045892];
regulation of circadian rhythm [GO:0042752];
regulation of transcription, DNA-templated [GO:0006355];
RNA splicing [GO:0008380];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
core promoter binding [GO:0001047];
identical protein binding [GO:0042802];
nucleotide binding [GO:0000166];
poly(A RNA binding [GO:0044822];
RNA polymerase II distal enhancer sequence-specific DNA binding [GO:0000980];
transcription regulatory region sequence-specific DNA binding [GO:0000976]
Gene Ontology
(Cellular Component)
Complete annatation
membrane [GO:0016020];
nuclear matrix [GO:0016363];
nuclear speck [GO:0016607];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
paraspeckles [GO:0042382];
RNA polymerase II transcription factor complex [GO:0090575]
Protein-protein interaction110904
Phylogenetic treeQ15233
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7002547563205640.03297998409855480.0703871878127186
AZA vs. DISU0.08380710866003510.7398725053632820.974754980471983
AZA vs. IL70.09050007810598750.6367567344454490.999311006273513
AZA vs. SAHA0.2242347421163890.3575153254383890.726381924459075
DISU vs. CD3-0.6288447925650310.08326612864355990.161600826982493
DISU vs. IL7-0.002745117974900220.9912826602515080.999236783904479
DISU vs. SAHA0.1423317891278990.6256265055671740.882215698850372
DMSO vs. AZA0.05825898300851550.7268383546282711
DMSO vs. CD3-0.6536143078606860.04137603331819210.08192008699416
DMSO vs. DISU-0.0275110211187380.9100068101900050.988283279918411
DMSO vs. IL70.03955628128143990.8252537351691010.963051812112721
DMSO vs. SAHA0.1599626870334670.4971486634496860.81246231911193
HIV vs. Mock in Activation0.0778436599640.900265517260190.999983755607037
HIV vs. Mock in Latency-0.02651177070455720.8718585726109270.999834320637052
IL7 vs. CD3-0.603051468818170.06081826135930180.128806189893039
SAHA vs. CD3-0.4998829043206190.1585051289985150.251381925713105
SAHA vs. IL70.1309773701938410.5910344143507860.791792815670436
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0180883 0.948852
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.012 1.1 0.986 0.993 1.134
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3SDE X-ray 1.9Å B=53-312.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) inhibited by 25769457
Rev interacts with 22174317
Envelope surface glycoprotein gp120 complexes with 23125841
retropepsin cleaves 22944692
Gag-Pol complexes with 23125841
Rev associates with 25589658
Vpr upregulates 23874603
Pr55(Gag) complexes with 23125841
capsid inhibited by 25769457
HIV-1 virus replication inhibited by expression of human gene 25769457
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found