Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001961
UniProt IDQ8NFZ4
Primary gene name(s)NLGN2
Synonym gene name(s)KIAA1366
Protein nameNeuroligin-2
Protein functionTransmembrane scaffolding protein involved in cell-cell interactions via its interactions with neurexin family members. Mediates cell-cell interactions both in neurons and in other types of cells, such as Langerhans beta cells. Plays a role in synapse function and synaptic signal transmission, especially via gamma-aminobutyric acid receptors, GABA(A receptors. Functions by recruiting and clustering synaptic proteins. Promotes clustering of postsynaptic GABRG2 and GPHN. Modulates signaling by inhibitory synapses, and thereby plays a role in controlling the ratio of signaling by excitatory and inhibitory synapses and information processing. Required for normal signal amplitude from inhibitory synapses, but is not essential for normal signal frequency. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. Mediates cell-cell interactions between Langerhans beta cells and modulates insulin secretion, By similarity. {ECO:0000250}.
Subcellular locationCell membrane {ECO:0000250};
Single-pass type I membrane protein {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane {ECO:0000250}. Cell junction, synapse, presynaptic cell membrane {ECO:0000250}. Note=Detected at postsynaptic membranes in brain. Detected at dendritic spines in cultured neurons. Colocalizes with GPHN and ARHGEF9 at neuronal cell membranes, By similarity. Localized at presynaptic membranes in retina. Colocalizes with GABRG2 at inhibitory synapses in the retina, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8NFZ4
Gene Ontology
(Biological Process)
Complete annatation
cell-cell junction maintenance [GO:0045217];
gephyrin clustering involved in postsynaptic density assembly [GO:0097116];
inhibitory synapse assembly [GO:1904862];
locomotory exploration behavior [GO:0035641];
modulation of synaptic transmission [GO:0050804];
neuromuscular process controlling balance [GO:0050885];
neuron cell-cell adhesion [GO:0007158];
positive regulation of excitatory postsynaptic potential [GO:2000463];
positive regulation of inhibitory postsynaptic potential [GO:0097151];
positive regulation of insulin secretion [GO:0032024];
positive regulation of protein localization to synapse [GO:1902474];
positive regulation of synapse assembly [GO:0051965];
positive regulation of synaptic transmission, GABAergic [GO:0032230];
positive regulation of synaptic transmission, glutamatergic [GO:0051968];
positive regulation of synaptic vesicle clustering [GO:2000809];
postsynaptic density protein 95 clustering [GO:0097119];
postsynaptic membrane assembly [GO:0097104];
presynaptic membrane assembly [GO:0097105];
protein localization to synapse [GO:0035418];
regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [GO:2000311];
regulation of respiratory gaseous exchange by neurological system process [GO:0002087];
sensory perception of pain [GO:0019233];
single organismal cell-cell adhesion [GO:0016337];
synapse assembly [GO:0007416];
synapse organization [GO:0050808];
terminal button organization [GO:0072553]
Gene Ontology
(Molecular Function)
Complete annatation
carboxylic ester hydrolase activity [GO:0052689];
cell adhesion molecule binding [GO:0050839];
neurexin family protein binding [GO:0042043];
receptor activity [GO:0004872]
Gene Ontology
(Cellular Component)
Complete annatation
cell junction [GO:0030054];
cell surface [GO:0009986];
inhibitory synapse [GO:0060077];
integral component of plasma membrane [GO:0005887];
membrane [GO:0016020];
postsynaptic membrane [GO:0045211];
presynaptic membrane [GO:0042734];
synapse [GO:0045202]
Protein-protein interaction121611
Phylogenetic treeQ8NFZ4
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.2988617674545150.3780354202256180.499671126338728
AZA vs. DISU1.1023663087657.28193814495404e-050.0130676113805997
AZA vs. IL7-0.1182271037769350.6909970082158410.999311006273513
AZA vs. SAHA2.057915794082723.49675510769032e-101.03353110771223e-07
DISU vs. CD30.7910431121970890.03270006412588210.0768148303932596
DISU vs. IL7-1.230239444788091.17532586333402e-050.000991645825001423
DISU vs. SAHA0.9592497074828350.001286370369543380.0246026495423709
DMSO vs. AZA0.05718275253353260.7909921062328251
DMSO vs. CD3-0.2535914634828050.4435151302463950.555633538060527
DMSO vs. DISU-1.047066532285894.28218415561288e-050.00816867281330204
DMSO vs. IL7-0.1666190555485770.4537669731083350.870758796926069
DMSO vs. SAHA1.997095650645875.6621374255883e-155.01965136123772e-12
HIV vs. Mock in Activation0.2680951343318980.6742569106291240.999983755607037
HIV vs. Mock in Latency0.1291977018746930.579105858283880.999834320637052
IL7 vs. CD3-0.413050689394570.213799510330270.339252510270561
SAHA vs. CD31.7369013436162.22970874386164e-061.66923550769969e-05
SAHA vs. IL72.173975296766364.54651871706346e-118.15883608821603e-09
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.532125 0.00154878
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04514 Cell adhesion molecules (CAMs) - Homo sapiens (human)