Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001946
UniProt IDQ9NX24
Primary gene name(s)NHP2
Synonym gene name(s)NOLA2
Protein nameH/ACA ribonucleoprotein complex subunit 2
Protein functionRequired for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein, H/ACA snoRNP complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine, ""psi"" residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase, TERT holoenzyme. {ECO:0000269|PubMed:15044956}."
Subcellular locationNucleus, nucleolus. Nucleus, Cajal body. Note=Also localized to Cajal bodies, coiled bodies.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NX24
Gene Ontology
(Biological Process)
Complete annatation
cleavage involved in rRNA processing [GO:0000469];
maturation of LSU-rRNA [GO:0000470];
positive regulation of telomerase RNA localization to Cajal body [GO:1904874];
rRNA pseudouridine synthesis [GO:0031118];
snRNA pseudouridine synthesis [GO:0031120];
telomere maintenance via telomerase [GO:0007004];
translation [GO:0006412]
Gene Ontology
(Molecular Function)
Complete annatation
box H/ACA snoRNA binding [GO:0034513];
poly(A RNA binding [GO:0044822];
snoRNA binding [GO:0030515];
telomerase RNA binding [GO:0070034]
Gene Ontology
(Cellular Component)
Complete annatation
box H/ACA scaRNP complex [GO:0072589];
box H/ACA snoRNP complex [GO:0031429];
box H/ACA telomerase RNP complex [GO:0090661];
cytoplasm [GO:0005737];
nuclear chromosome, telomeric region [GO:0000784];
nucleoplasm [GO:0005654];
small nucleolar ribonucleoprotein complex [GO:0005732];
telomerase holoenzyme complex [GO:0005697]
Protein-protein interaction120783
Phylogenetic treeQ9NX24
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.349655816387315.06338492479719e-050.000264458870047582
AZA vs. DISU-0.5331922708254040.07116525526130090.578611142291721
AZA vs. IL70.4771518048629270.0136932423057530.388871750448342
AZA vs. SAHA-0.5262367796005370.03276543620383210.209508822349925
DISU vs. CD3-1.894544003159664.36012876914305e-074.59612455007428e-06
DISU vs. IL71.000669294008940.0003082546955230910.0122425753485249
DISU vs. SAHA0.008886974531870050.9760227823847330.995922620191181
DMSO vs. AZA0.04398248149154470.7946294965991471
DMSO vs. CD3-1.317345810014265.0862248586192e-050.000246221007374995
DMSO vs. DISU0.575077497490720.03638283217751970.384764407659805
DMSO vs. IL70.4405836684846880.01487231811623080.255641803944267
DMSO vs. SAHA-0.5757861974790040.01545914719292950.119418148305813
HIV vs. Mock in Activation-0.08717322196077570.8887951170443530.999983755607037
HIV vs. Mock in Latency-0.08838575227088620.5969556808166430.999834320637052
IL7 vs. CD3-0.8658776301786750.007449080282749150.0234453930051948
SAHA vs. CD3-1.89919637523372.15427947480862e-072.06651761205384e-06
SAHA vs. IL7-1.00560446513484.64263919098817e-050.00106035065401448
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.231457 0.0931871
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.006 0.977 0.895 0.812 0.944
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin cleaves 22944692

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03008 Ribosome biogenesis in eukaryotes - Homo sapiens (human)