Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001937
UniProt IDQ8NCF5
Primary gene name(s)NFATC2IP
Synonym gene name(s)NIP45
Protein nameNFATC2-interacting protein
Protein functionIn T-helper 2, Th2 cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2. Recruits PRMT1 to the IL4 promoter; this leads to enhancement of histone H4 'Arg-3'-methylation and facilitates subsequent histone acetylation at the IL4 locus, thus promotes robust cytokine expression, By similarity. Down-regulates formation of poly-SUMO chains by UBE2I/UBC9, By similarity. {ECO:0000250}.
Subcellular locationNucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. Note=TRAF1 is associated with a fraction of NFATC2IP in the cytoplasm and prevents its translocation to the nucleus. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8NCF5
Gene Ontology
(Biological Process)
Complete annatation
cytokine production [GO:0001816];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
protein sumoylation [GO:0016925]
Gene Ontology
(Molecular Function)
Complete annatation
protein tag [GO:0031386]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nucleus [GO:0005634]
Protein-protein interaction124341
Phylogenetic treeQ8NCF5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.03002446734551910.9268350460020670.952566429472334
AZA vs. DISU-0.03200678726541340.8991754297143530.993937521279788
AZA vs. IL7-0.09282427728627430.628871466503590.999311006273513
AZA vs. SAHA-0.2707088541915840.2670155495997170.640381335756949
DISU vs. CD3-0.0153534639839830.9661576038501210.97875401347021
DISU vs. IL7-0.07000371293665520.7808400082576360.94950939101024
DISU vs. SAHA-0.2362821971371990.4171460052832940.771430478236783
DMSO vs. AZA0.0003597713391590510.9982841684547321
DMSO vs. CD30.01937860828713420.9516041485878530.966032109061726
DMSO vs. DISU0.03094252904532350.8989459631561190.988283279918411
DMSO vs. IL7-0.08603468778247060.6319503428566720.920378169311359
DMSO vs. SAHA-0.2768545870112140.2401227533569210.588437400949944
HIV vs. Mock in Activation0.1404825929532120.8212010605660990.999983755607037
HIV vs. Mock in Latency0.02252719203334230.891237400284760.999834320637052
IL7 vs. CD3-0.05671347366221870.8596989772253790.910564449702631
SAHA vs. CD3-0.2643782640339020.4539299497616140.566562572287389
SAHA vs. IL7-0.1808358761095810.457602755992050.694583017201103
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.318955 0.59665
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.064 1.213 1.021 1.004 1.074
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2JXX NMR - A=342-419.
2L76 NMR - A=244-338.
3RD2 X-ray 1.6Å A=345-419.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found