Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001936
UniProt IDO94916
Primary gene name(s)NFAT5
Synonym gene name(s)KIAA0827, TONEBP
Protein nameNuclear factor of activated T-cells 5
Protein functionTranscription factor involved in the transcriptional regulation of osmoprotective and inflammatory genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.
Subcellular locationNucleus. Cytoplasm {ECO:0000250}. Note=Nuclear distribution increases under hypertonic conditions. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O94916
Gene Ontology
(Biological Process)
Complete annatation
cytokine production [GO:0001816];
excretion [GO:0007588];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
regulation of calcineurin-NFAT signaling cascade [GO:0070884];
response to osmotic stress [GO:0006970];
signal transduction [GO:0007165];
transcription from RNA polymerase II promoter [GO:0006366]
Gene Ontology
(Molecular Function)
Complete annatation
RNA polymerase II core promoter proximal region sequence-specific DNA binding [GO:0000978];
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding [GO:0001077];
transcription factor activity, sequence-specific DNA binding [GO:0003700]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction115949
Phylogenetic treeO94916
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.018873798728930.002234429850218270.00728473430726578
AZA vs. DISU0.3279555727123760.1948989997313470.78430985033964
AZA vs. IL7-0.08668348058846250.652265788792540.999311006273513
AZA vs. SAHA0.3579155953119880.144698325993360.484384314018191
DISU vs. CD3-0.7056989224069130.05480392074161840.116354126937909
DISU vs. IL7-0.4230429452998490.09347876041098570.406256356932317
DISU vs. SAHA0.03117079384219040.9152187295239660.981028554242936
DMSO vs. AZA0.06943937926617480.6786975405707651
DMSO vs. CD3-0.9620564209697040.003036273025410760.00899183273595698
DMSO vs. DISU-0.2606062319948280.2854414098386490.798828339966565
DMSO vs. IL7-0.1486850323234330.4085152910018230.847494592253841
DMSO vs. SAHA0.2812725180003390.2347000787371740.581789451798405
HIV vs. Mock in Activation0.5235629756837850.4360054500174410.999983755607037
HIV vs. Mock in Latency0.02761929271770130.8670627034023380.999834320637052
IL7 vs. CD3-1.097592894474180.0007883053620731940.00346417688703477
SAHA vs. CD3-0.687143189980730.05259763390404970.103222599678868
SAHA vs. IL70.4396879843200690.07248226806809350.235068353885207
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.413 0.02865

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.327741 0.0171733
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1IMH X-ray 2.8Å C/D=264-544.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpu regulates 25728138
Nef regulates 25728138
retropepsin interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found