Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001929
UniProt IDQ8TAT5
Primary gene name(s)NEIL3
Synonym gene name(s)unknown
Protein nameEndonuclease 8-like 3
Protein functionDNA glycosylase which prefers single-stranded DNA, ssDNA, or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA, dsDNA. In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin, Sp and guanidinohydantoin, Gh in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair. {ECO:0000269|PubMed:12433996, ECO:0000269|PubMed:19170771, ECO:0000269|PubMed:22569481, ECO:0000269|PubMed:23755964}.
Subcellular locationNucleus {ECO:0000269|PubMed:12433996}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8TAT5
Gene Ontology
(Biological Process)
Complete annatation
base-excision repair [GO:0006284];
nucleotide-excision repair [GO:0006289]
Gene Ontology
(Molecular Function)
Complete annatation
bubble DNA binding [GO:0000405];
damaged DNA binding [GO:0003684];
DNA-(apurinic or apyrimidinic site lyase activity [GO:0003906];
DNA N-glycosylase activity [GO:0019104];
double-stranded DNA binding [GO:0003690];
single-stranded DNA binding [GO:0003697];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction120538
Phylogenetic treeQ8TAT5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Role of the gene in HIV infection is validated by cDNA rescue
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3376043477201480.8405363012869090.893032788171175
AZA vs. DISU0.1918128864965370.5949967850601050.955158276076142
AZA vs. IL70.6035997948324130.1855045514502490.952505417553304
AZA vs. SAHA-0.3519727131467810.3503206301305840.721371733622619
DISU vs. CD30.5201614350202330.7596853423711730.833260339875068
DISU vs. IL70.4005116182889120.3247837742042280.705390490725937
DISU vs. SAHA-0.5444296882335570.0852819239112830.372330207203634
DMSO vs. AZA-0.2191553821825040.5373821722200651
DMSO vs. CD30.1062476451360320.950694460675750.965498992185531
DMSO vs. DISU-0.4128527513416240.1461424137501770.640630434897754
DMSO vs. IL70.8283130728086330.03967215602376160.407247887015197
DMSO vs. SAHA-0.1415201927613220.6411769584857910.884178233685345
HIV vs. Mock in Activation0.1042368704777870.9672191624177250.999983755607037
HIV vs. Mock in Latency0.28777864897110.5398896848586870.999834320637052
IL7 vs. CD30.9534975525677260.5629661672904980.684309461162578
SAHA vs. CD3-0.03797893795697160.9822630897568070.987900744970307
SAHA vs. IL7-0.9586487966515270.02334628745186850.111036456440315
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.292137 0.0514143
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03410 Base excision repair - Homo sapiens (human)