Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001928
UniProt IDQ14511
Primary gene name(s)NEDD9
Synonym gene name(s)CASL, CASS2
Protein nameEnhancer of filamentation 1
Protein functionDocking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor, BCR mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.
Subcellular locationCytoplasm, cell cortex. Nucleus. Golgi apparatus. Cell projection, lamellipodium. Cytoplasm. Cell junction, focal adhesion. Note=Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle.;
SUBCELLULAR LOCATION: Enhancer of filamentation 1 p55: Cytoplasm, cytoskeleton, spindle.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q14511
Gene Ontology
(Biological Process)
Complete annatation
actin filament bundle assembly [GO:0051017];
cell adhesion [GO:0007155];
cell division [GO:0051301];
cytoskeleton organization [GO:0007010];
integrin-mediated signaling pathway [GO:0007229];
mitotic nuclear division [GO:0007067];
regulation of growth [GO:0040008];
signal transduction [GO:0007165]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
cell cortex [GO:0005938];
cytoplasm [GO:0005737];
focal adhesion [GO:0005925];
Golgi apparatus [GO:0005794];
lamellipodium [GO:0030027];
nucleus [GO:0005634];
spindle [GO:0005819];
spindle pole [GO:0000922]
Protein-protein interaction110816
Phylogenetic treeQ14511
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1290537265515180.6955878043326210.782614167430671
AZA vs. DISU-0.3095559831406940.2436352378691120.827346002615447
AZA vs. IL70.4515117412795390.01912329064867990.446508985160725
AZA vs. SAHA-0.3633792782748480.1473640392411910.488426896992463
DISU vs. CD3-0.1916390641191270.5980699623970660.708979972761095
DISU vs. IL70.7509846121505990.003057931574623090.0559689745042763
DISU vs. SAHA-0.05092783422272080.8640247166161460.963215955627039
DMSO vs. AZA-0.02302295154635880.9182594304031231
DMSO vs. CD30.1000585172954060.7620759835851550.829228941329748
DMSO vs. DISU0.2862291290337790.3079339402461520.812857137262187
DMSO vs. IL70.4811404713403140.01068326638740490.215885041273631
DMSO vs. SAHA-0.3465393691446110.1943761977919410.531273789793678
HIV vs. Mock in Activation0.4920303005496140.4299162875402860.999983755607037
HIV vs. Mock in Latency0.06257848540357950.7572675500634680.999834320637052
IL7 vs. CD30.5851015443988280.07186293926088090.146535455016133
SAHA vs. CD3-0.2561591171625140.4699115004324550.581415351947459
SAHA vs. IL7-0.8156175724974160.0008902991719891280.0106935216880989
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock 0.83 1.13E-11 3.20E-09
Infected vs. Bystander 0.976 3.75E-13 1.27E-10
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.260712 0.0588325
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2L81 NMR - A=399-563.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
capsid interacts with 24663101

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found