Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001919
UniProt IDQ9GZM8
Primary gene name(s)NDEL1
Synonym gene name(s)EOPA, MITAP1, NUDEL
Protein nameNuclear distribution protein nudE-like 1
Protein functionRequired for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. {ECO:0000269|PubMed:12556484, ECO:0000269|PubMed:14970193, ECO:0000269|PubMed:16291865, ECO:0000269|PubMed:17600710}.
Subcellular locationCytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome, By similarity. Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9GZM8
Gene Ontology
(Biological Process)
Complete annatation
activation of GTPase activity [GO:0090630];
cell migration [GO:0016477];
central nervous system neuron axonogenesis [GO:0021955];
centrosome localization [GO:0051642];
cerebral cortex radially oriented cell migration [GO:0021799];
chromosome segregation [GO:0007059];
establishment of chromosome localization [GO:0051303];
establishment of mitotic spindle orientation [GO:0000132];
inner cell mass cell proliferation [GO:0001833];
microtubule nucleation [GO:0007020];
mitotic centrosome separation [GO:0007100];
neurofilament cytoskeleton organization [GO:0060052];
neuron migration [GO:0001764];
neuron projection extension [GO:1990138];
nuclear envelope disassembly [GO:0051081];
positive regulation of axon extension [GO:0045773];
positive regulation of axon regeneration [GO:0048680];
positive regulation of GTPase activity [GO:0043547];
regulation of intracellular protein transport [GO:0033157];
regulation of microtubule motor activity [GO:2000574];
regulation of neuron projection development [GO:0010975];
retrograde axonal transport [GO:0008090];
sister chromatid cohesion [GO:0007062];
vesicle transport along microtubule [GO:0047496]
Gene Ontology
(Molecular Function)
Complete annatation
microtubule binding [GO:0008017];
oligopeptidase activity [GO:0070012]
Gene Ontology
(Cellular Component)
Complete annatation
axon cytoplasm [GO:1904115];
axon hillock [GO:0043203];
cell leading edge [GO:0031252];
centrosome [GO:0005813];
condensed chromosome kinetochore [GO:0000777];
cytosol [GO:0005829];
kinesin complex [GO:0005871];
kinetochore [GO:0000776];
microtubule [GO:0005874];
neurofilament cytoskeleton [GO:0060053];
nuclear envelope [GO:0005635];
spindle [GO:0005819];
synaptic vesicle [GO:0008021]
Protein-protein interaction123527
Phylogenetic treeQ9GZM8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1893220813566050.5633719579785130.672618991654954
AZA vs. DISU0.1787637082274560.4812428589729650.931377782001655
AZA vs. IL7-0.004944452135925670.9795544799987870.999311006273513
AZA vs. SAHA-0.1207199857941810.6214266467374650.878411972329383
DISU vs. CD3-0.02283909644691350.9499612761312480.967352312126085
DISU vs. IL7-0.1931635481277470.4451488592033130.793177945210925
DISU vs. SAHA-0.297772523854050.3086498457668890.692259502858335
DMSO vs. AZA-0.03812205662834670.8209091341837541
DMSO vs. CD3-0.237288174499150.4584635909971530.569817176666291
DMSO vs. DISU-0.2183240115596080.3724447531208840.852590870876848
DMSO vs. IL70.04028117822698320.8234130404701750.963051812112721
DMSO vs. SAHA-0.08919800554942560.7057168675480880.911755337604151
HIV vs. Mock in Activation0.1793275292562650.7731973240749870.999983755607037
HIV vs. Mock in Latency0.021765551450020.8955084924121150.999834320637052
IL7 vs. CD3-0.187774063503350.5589963900983220.680813880733032
SAHA vs. CD3-0.3341802666231280.3446613073232190.460748486215395
SAHA vs. IL7-0.1186305857331590.6267713832738220.814548825887541
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.191197 0.190592
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2V66 X-ray 2.1Å B/C/D/E=58-168.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found