Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001915
UniProt IDQ9Y6Q9
Primary gene name(s)NCOA3
Synonym gene name(s)AIB1, BHLHE42, RAC3, TRAM1
Protein nameNuclear receptor coactivator 3
Protein functionNuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids, GR and ER, retinoids, RARs and RXRs, thyroid hormone, TRs, vitamin D3, VDR and prostanoids, PPARs. Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
Subcellular locationCytoplasm. Nucleus. Note=Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y6Q9
Gene Ontology
(Biological Process)
Complete annatation
androgen receptor signaling pathway [GO:0030521];
cellular response to estradiol stimulus [GO:0071392];
intracellular receptor signaling pathway [GO:0030522];
positive regulation of gene expression [GO:0010628];
positive regulation of keratinocyte differentiation [GO:0045618];
positive regulation of transcription, DNA-templated [GO:0045893];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
receptor transactivation [GO:0035624];
regulation of RNA biosynthetic process [GO:2001141];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
androgen receptor binding [GO:0050681];
histone acetyltransferase activity [GO:0004402];
ligand-dependent nuclear receptor binding [GO:0016922];
ligand-dependent nuclear receptor transcription coactivator activity [GO:0030374];
nuclear hormone receptor binding [GO:0035257];
protein N-terminus binding [GO:0047485];
thyroid hormone receptor binding [GO:0046966];
transcription coactivator activity [GO:0003713]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
nuclear chromatin [GO:0000790];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction113841
Phylogenetic treeQ9Y6Q9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.397099374757340.2261911230338690.335546516374653
AZA vs. DISU0.406429129516620.1096564594414450.656320299699601
AZA vs. IL70.2454743503674030.2010048891939660.965934195269828
AZA vs. SAHA0.05882745372579540.8092055109931260.95147924124106
DISU vs. CD3-0.003307180257507540.9927863257679250.995427768699329
DISU vs. IL7-0.1699682381452510.5006374630091430.826627574464713
DISU vs. SAHA-0.3460423217933060.238395388407620.61664083952142
DMSO vs. AZA-0.00411267800007370.9803720523147331
DMSO vs. CD3-0.4107770620647470.2001941604415530.299287985219013
DMSO vs. DISU-0.4118454724479330.09261299130648590.558047892438522
DMSO vs. IL70.256588899467790.1529354708113410.655903903977513
DMSO vs. SAHA0.0557816885023790.8127756072356620.948464420150524
HIV vs. Mock in Activation0.06787313743417680.913833994638240.999983755607037
HIV vs. Mock in Latency-0.01379592998418570.933382033154490.999834320637052
IL7 vs. CD3-0.1441903425185020.6541871634638640.762139674979968
SAHA vs. CD3-0.3626223164255860.3047633332408120.418248195240783
SAHA vs. IL7-0.1897729925940120.4355968161821870.676438282386967
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.105774 0.500527
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.074 0.885 1.021 0.997 0.859
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1KBH NMR - A=1045-1091.
3L3X X-ray 1.5Å B=618-629.
3L3Z X-ray 2.0Å B=735-746.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat stimulates 11704662
nucleocapsid downregulates 19884766
Tat enhanced by 11704662
Tat downregulates 19884766
HIV-1 virus replication enhanced by expression of human gene 19460752

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa01522 Endocrine resistance - Homo sapiens (human)
hsa04919 Thyroid hormone signaling pathway - Homo sapiens (human)
hsa05224 Breast cancer - Homo sapiens (human)