Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001912
UniProt IDQ6PIU2
Primary gene name(s)NCEH1
Synonym gene name(s)AADACL1, KIAA1363
Protein nameNeutral cholesterol ester hydrolase 1
Protein functionHydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration. {ECO:0000269|PubMed:17052608}.
Subcellular locationMembrane {ECO:0000305|PubMed:12149457};
Single-pass type II membrane protein {ECO:0000305|PubMed:12149457}. Microsome {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q6PIU2
Gene Ontology
(Biological Process)
Complete annatation
catabolic process [GO:0009056];
lipid catabolic process [GO:0016042];
xenobiotic metabolic process [GO:0006805]
Gene Ontology
(Molecular Function)
Complete annatation
sterol esterase activity [GO:0004771]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum membrane [GO:0005789];
integral component of membrane [GO:0016021];
membrane [GO:0016020]
Protein-protein interaction121608
Phylogenetic treeQ6PIU2
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.4798285394590770.2330116350953920.343098023071293
AZA vs. DISU-0.1300183148002910.7162879194316430.973233942294547
AZA vs. IL7-0.160102662763240.6069989325916420.999311006273513
AZA vs. SAHA0.1534005189221190.5785099877930750.860331614464339
DISU vs. CD3-0.6211778903508340.130388372645470.229477446498285
DISU vs. IL7-0.03924335219755490.9033865580108790.982189124739072
DISU vs. SAHA0.2835814868020970.3431892754342310.721487104751149
DMSO vs. AZA-0.0917624826279310.7750503337159191
DMSO vs. CD3-0.5861722315653180.1209857493426940.199131466606324
DMSO vs. DISU0.03499770102957850.9154909297531640.988576460318632
DMSO vs. IL7-0.06044270678303060.8286452419378090.963051812112721
DMSO vs. SAHA0.2381110736924550.3267091082017160.680691095710236
HIV vs. Mock in Activation0.1159877051284180.8809266073519360.999983755607037
HIV vs. Mock in Latency0.002016440620591270.9908063111056560.999834320637052
IL7 vs. CD3-0.6309945392600080.09783474943554760.186548156640355
SAHA vs. CD3-0.352165547734220.3330257447973890.448298166511537
SAHA vs. IL70.3101930260863230.2161960695497820.454435476073282
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock 0.912 8.99E-12 2.78E-09
Infected vs. Bystander 1.021 8.47E-13 2.31E-10
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.648345 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.004 1.156 1.29 1.415 1.215
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat downregulates 23898208

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04976 Bile secretion - Homo sapiens (human)