Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001903
UniProt IDQ9H0A0
Primary gene name(s)NAT10
Synonym gene name(s)ALP, KIAA1709
Protein nameRNA cytidine acetyltransferase
Protein functionRNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs, PubMed:25653167. Catalyzes the formation of N(4-acetylcytidine, ac4C at positions 1337 and 1842 in 18S rRNA, By similarity. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis, PubMed:25653167. Catalyzes the formation of ac4C in serine and leucine tRNAs, By similarity. Requires the tRNA-binding adapter protein THUMBD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation, PubMed:25653167. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division, PubMed:14592445, PubMed:17631499, PubMed:18082603, PubMed:19303003. {ECO:0000250|UniProtKB:P53914, ECO:0000255|HAMAP-Rule:MF_03211, ECO:0000269|PubMed:14592445, ECO:0000269|PubMed:17631499, ECO:0000269|PubMed:18082603, ECO:0000269|PubMed:19303003, ECO:0000269|PubMed:25653167}.
Subcellular locationNucleus, nucleolus {ECO:0000255|HAMAP-Rule:MF_03211, ECO:0000269|PubMed:12429849, ECO:0000269|PubMed:14592445, ECO:0000269|PubMed:19303003, ECO:0000269|PubMed:25653167}. Note=Nucleolar in interphase and redistributes to the perichromosomal layer and to the midbody during telophase. {ECO:0000269|PubMed:19303003}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9H0A0
Gene Ontology
(Biological Process)
Complete annatation
negative regulation of telomere maintenance via telomerase [GO:0032211];
ribosomal small subunit biogenesis [GO:0042274];
rRNA modification [GO:0000154];
tRNA acetylation [GO:0051391]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
N-acetyltransferase activity [GO:0008080];
poly(A RNA binding [GO:0044822]
Gene Ontology
(Cellular Component)
Complete annatation
membrane [GO:0016020];
nuclear chromosome, telomeric region [GO:0000784];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction120521
Phylogenetic treeQ9H0A0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.138592717575380.0006610014663317450.00252858225902337
AZA vs. DISU-0.2234975658301680.3772889982276760.898589466327254
AZA vs. IL70.3470766961407910.07131921530347220.750344346938375
AZA vs. SAHA-0.2055611892053870.3996985255254380.759421614495493
DISU vs. CD3-1.373844861220990.0002012947226543990.00104307894441128
DISU vs. IL70.5613240653105340.02634300211722040.207265530750051
DISU vs. SAHA0.01953487790766460.946593470557560.98809462493233
DMSO vs. AZA0.05560655772109150.7401883427193081
DMSO vs. CD3-1.094148514712050.0008404921670245310.00294055143897459
DMSO vs. DISU0.2773127923191060.2564383870925370.774203834663033
DMSO vs. IL70.2988077392489130.09675688341312380.56912520641965
DMSO vs. SAHA-0.2673795658030120.2569948360080180.605739507455754
HIV vs. Mock in Activation0.09149588075635140.8971627305036420.999983755607037
HIV vs. Mock in Latency-0.02627532120566960.8738020993806280.999834320637052
IL7 vs. CD3-0.7845090446678810.0158024350953150.0433388108063469
SAHA vs. CD3-1.368137522233350.0001555776069943620.000706191125724406
SAHA vs. IL7-0.5555085771705040.022882841039040.109677566239265
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.00773551 0.972245
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.987 0.99 0.801 0.63 0.754
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Rev interacts with 22174317
Pr55(Gag) interacts with 25010285
26362536

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03008 Ribosome biogenesis in eukaryotes - Homo sapiens (human)
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