Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001902
UniProt IDP49321
Primary gene name(s)NASP
Synonym gene name(s)unknown
Protein nameNuclear autoantigenic sperm protein
Protein functionRequired for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA, By similarity. {ECO:0000250}.
Subcellular locationCytoplasm {ECO:0000250}. Nucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P49321
Gene Ontology
(Biological Process)
Complete annatation
blastocyst development [GO:0001824];
cell cycle [GO:0007049];
cell proliferation [GO:0008283];
DNA replication [GO:0006260];
DNA replication-dependent nucleosome assembly [GO:0006335];
DNA replication-independent nucleosome assembly [GO:0006336];
histone exchange [GO:0043486];
male gonad development [GO:0008584];
nucleosome assembly [GO:0006334];
protein transport [GO:0015031];
response to testosterone [GO:0033574]
Gene Ontology
(Molecular Function)
Complete annatation
Hsp90 protein binding [GO:0051879];
protein complex binding [GO:0032403]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nuclear chromatin [GO:0000790];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
protein complex [GO:0043234]
Protein-protein interaction110759
Phylogenetic treeP49321
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.78583394127131.32517343542915e-071.242875794573e-06
AZA vs. DISU0.04273722608205950.8668808239764380.991211477030548
AZA vs. IL70.4708208365932320.01472721731808770.402312723755907
AZA vs. SAHA-0.1558079724970710.5233559945699120.835087145352689
DISU vs. CD3-1.75536113876642.44432721374643e-062.12981435953837e-05
DISU vs. IL70.4191497841462620.09733931160054790.4124199332148
DISU vs. SAHA-0.197230901239280.5017238795973090.826423099272022
DMSO vs. AZA-0.01498980246911690.9288583509552691
DMSO vs. CD3-1.81102701037614.36636142797653e-084.09329402068864e-07
DMSO vs. DISU-0.05919312495527420.809763212610110.978675093908237
DMSO vs. IL70.4928067232080080.006347441940019680.165263708541963
DMSO vs. SAHA-0.1479365489927130.5306034583724840.829293439441583
HIV vs. Mock in Activation-0.01082905731321530.9860946121716220.999983755607037
HIV vs. Mock in Latency0.03921085975214850.8124789165414410.999834320637052
IL7 vs. CD3-1.307013169266636.20248375896582e-050.000384258272498527
SAHA vs. CD3-1.966000779609418.63686917540463e-089.23758825218933e-07
SAHA vs. IL7-0.6296291151136480.01011080435987790.0629990164361017
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change -1.07055852
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.503865 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.044 0.915 0.857 0.859 0.95
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
201969_at 1.42 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin cleaves 22944692
Tat upregulates 23364796

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found