Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001893
UniProt IDQ13564
Primary gene name(s)NAE1
Synonym gene name(s)APPBP1
Protein nameNEDD8-activating enzyme E1 regulatory subunit
Protein functionRegulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. {ECO:0000269|PubMed:10207026, ECO:0000269|PubMed:10722740, ECO:0000269|PubMed:12740388}.
Subcellular locationCell membrane {ECO:0000269|PubMed:14557245}. Note=Colocalizes with APP in lipid rafts.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13564
Gene Ontology
(Biological Process)
Complete annatation
mitotic DNA replication checkpoint [GO:0033314];
neuron apoptotic process [GO:0051402];
protein neddylation [GO:0045116];
regulation of apoptotic process [GO:0042981];
regulation of neuron apoptotic process [GO:0043523];
signal transduction [GO:0007165]
Gene Ontology
(Molecular Function)
Complete annatation
NEDD8 activating enzyme activity [GO:0019781];
protein heterodimerization activity [GO:0046982];
ubiquitin protein ligase binding [GO:0031625]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction114402
Phylogenetic treeQ13564
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9199144283597050.005567930834012190.0158638664518396
AZA vs. DISU-0.07174687392512780.7769537317708520.976444104025318
AZA vs. IL70.123508002456180.5215660715854410.999311006273513
AZA vs. SAHA-0.1829746471994810.454567383716720.795842068156414
DISU vs. CD3-1.004555187754670.00615365937256840.0193215536587088
DISU vs. IL70.1865012650970320.4595870218324380.801883605801389
DISU vs. SAHA-0.1103342136211060.7051355224112810.91288546904155
DMSO vs. AZA-0.08685255172613430.6056291369304961
DMSO vs. CD3-1.018977731911240.001716623535517820.00545359206296416
DMSO vs. DISU-0.01705563412294330.9443663778301350.992938081932553
DMSO vs. IL70.2176238378119060.2275800672789240.735469251086859
DMSO vs. SAHA-0.1026912847793910.6641957213260090.894876963713954
HIV vs. Mock in Activation-0.2829545948646890.6493553516867350.999983755607037
HIV vs. Mock in Latency0.005457646485349530.9737027648655270.999834320637052
IL7 vs. CD3-0.7877848470017590.01519644501098920.0420209164649862
SAHA vs. CD3-1.127937243786340.001626956593827060.00542678970096807
SAHA vs. IL7-0.3101703606945120.204035085223080.437589255462325
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.415237 0.0108507
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.98 1.041 1.046 1.089 1.12
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00171 Adenosine triphosphate approved, nutraceutical unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1R4M X-ray 3.0Å A/C/E/G=1-534.
1R4N X-ray 3.6Å A/C/E/G=1-534.
1TT5 X-ray 2.6Å A/C=1-534.
1YOV X-ray 2.6Å A/C=1-534.
2NVU X-ray 2.8Å A=1-534.
3DBH X-ray 2.8Å A/C/E/G=1-534.
3DBL X-ray 2.9Å A/C/E/G=1-534.
3DBR X-ray 3.0Å A/C/E/G=1-534.
3GZN X-ray 3.0Å A/C=1-534.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpu depends on 26215564

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa05010 Alzheimer's disease - Homo sapiens (human)