Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001890
UniProt IDQ13765
Primary gene name(s)NACA
Synonym gene name(s)unknown
Protein nameNascent polypeptide-associated complex subunit alpha
Protein functionPrevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum, ER. Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle, SRP, which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane, M sites. May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters. {ECO:0000269|PubMed:10982809, ECO:0000269|PubMed:15784678, ECO:0000269|PubMed:9877153}.
Subcellular locationCytoplasm. Nucleus. Note=The heterodimer is located mainly in the cytosol, and the homodimer in the nucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13765
Gene Ontology
(Biological Process)
Complete annatation
cardiac ventricle development [GO:0003231];
heart trabecula morphogenesis [GO:0061384];
negative regulation of striated muscle cell apoptotic process [GO:0010664];
negative regulation of transcription from RNA polymerase II promoter involved in heart development [GO:1901227];
positive regulation of cell proliferation involved in heart morphogenesis [GO:2000138];
positive regulation of skeletal muscle tissue growth [GO:0048633];
positive regulation of transcription from RNA polymerase II promoter involved in heart development [GO:1901228];
protein transport [GO:0015031];
regulation of skeletal muscle fiber development [GO:0048742];
skeletal muscle tissue regeneration [GO:0043403];
transcription, DNA-templated [GO:0006351];
translation [GO:0006412];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
transcription coactivator activity [GO:0003713]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
nascent polypeptide-associated complex [GO:0005854];
nucleus [GO:0005634]
Protein-protein interaction110748
Phylogenetic treeQ13765
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1020181526598950.7548694098772910.829921346974289
AZA vs. DISU-0.1275687667012120.6130014464589470.957895177103442
AZA vs. IL7-0.05982257658259570.7546271650502880.999311006273513
AZA vs. SAHA-0.1680141868635950.4896645312793370.814333153597244
DISU vs. CD3-0.03841774451733550.9154020115364370.945185966292229
DISU vs. IL70.05887752700202280.8146875665460210.960611268336431
DISU vs. SAHA-0.0394994890083490.8918889436375390.973232744788018
DMSO vs. AZA-0.1197511578253870.4722386773773961
DMSO vs. CD3-0.03151216386498960.9213352266112050.945949375649002
DMSO vs. DISU0.005303021166744350.9826068694706120.99735766500781
DMSO vs. IL70.06748518932604450.7061422809501410.940121322945024
DMSO vs. SAHA-0.05465631680272170.8160784415057760.950316156432875
HIV vs. Mock in Activation-0.1315552248677920.8326123059088150.999983755607037
HIV vs. Mock in Latency-0.1352741236970820.4099137807520940.999834320637052
IL7 vs. CD30.05063045640692060.874549355555880.919607050017367
SAHA vs. CD3-0.09142154641484960.7953739223176060.854210733275821
SAHA vs. IL7-0.1121310215332530.6442097364660480.82499048642809
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.668115 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.971 0.905 0.795 0.754 0.919
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3LKX X-ray 2.5Å B=84-136.
3MCB X-ray 1.9Å A=79-132.
3MCE X-ray 2.4Å A/B/C/D=81-133.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 downregulates 17676665

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found