Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001884
UniProt IDQ13459
Primary gene name(s)MYO9B
Synonym gene name(s)MYR5
Protein nameUnconventional myosin-IXb
Protein functionMyosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions, PubMed:9490638. Also acts as a GTPase activator for RHOA, PubMed:9490638, PubMed:26529257. Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1; interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA, PubMed:26529257. {ECO:0000269|PubMed:26529257, ECO:0000269|PubMed:9490638}.
Subcellular locationCytoplasm, cell cortex {ECO:0000269|PubMed:8907710}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:8907710}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:8907710, ECO:0000269|PubMed:9490638}. Note=In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region. {ECO:0000269|PubMed:8907710}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13459
Gene Ontology
(Biological Process)
Complete annatation
actin filament-based movement [GO:0030048];
regulation of Rho protein signal transduction [GO:0035023];
regulation of small GTPase mediated signal transduction [GO:0051056];
Rho protein signal transduction [GO:0007266];
Roundabout signaling pathway [GO:0035385]
Gene Ontology
(Molecular Function)
Complete annatation
actin binding [GO:0003779];
actin-dependent ATPase activity [GO:0030898];
ADP binding [GO:0043531];
ATPase activity [GO:0016887];
ATP binding [GO:0005524];
calmodulin binding [GO:0005516];
GTPase activator activity [GO:0005096];
microfilament motor activity [GO:0000146];
Rho GTPase binding [GO:0017048];
Roundabout binding [GO:0048495];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
actin cytoskeleton [GO:0015629];
actin filament [GO:0005884];
cell cortex [GO:0005938];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
lamellipodium [GO:0030027];
membrane [GO:0016020];
myosin complex [GO:0016459];
perinuclear region of cytoplasm [GO:0048471];
ruffle [GO:0001726]
Protein-protein interaction110734
Phylogenetic treeQ13459
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.9126687113146820.005644808809870060.0160586180412158
AZA vs. DISU0.09686114764973860.701652688012280.970369120483745
AZA vs. IL70.01520622599197190.9367927573266160.999311006273513
AZA vs. SAHA-0.3668821207850550.1324376668535790.463408864937525
DISU vs. CD30.9969435926862740.00637077171759870.0198826113604726
DISU vs. IL7-0.09109317589403540.7172628848703990.927828686711634
DISU vs. SAHA-0.4609580195187390.1149948392689580.435692194021398
DMSO vs. AZA0.06296235490439150.7061208341408291
DMSO vs. CD30.9656052652616930.002725637869902870.00817962838846351
DMSO vs. DISU-0.03528157337412130.8848644210572930.987802141379617
DMSO vs. IL7-0.04057823570740550.8209323117317790.962956477091318
DMSO vs. SAHA-0.4355102765977850.06489818609273960.294691341549767
HIV vs. Mock in Activation0.3374514078798370.5876464793171730.999983755607037
HIV vs. Mock in Latency0.1468406395421480.3724509563128650.999834320637052
IL7 vs. CD30.933404439622090.003903557454805860.013629446726034
SAHA vs. CD30.5228586912249270.1400567880856560.228154339687692
SAHA vs. IL7-0.3845974334775470.1145138493578160.311810289960209
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.00866696 0.973342
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.037 0.964 1.041 1.092 1.066
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
5C5S X-ray 2.2Å A/B/C/D=1691-1916.
5HPY X-ray 2.4Å A/D=1691-1916.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found