Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001882
UniProt IDQ9Y4I1
Primary gene name(s)MYO5A
Synonym gene name(s)MYH12
Protein nameUnconventional myosin-Va
Protein functionProcessive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation. {ECO:0000269|PubMed:10448864}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y4I1
Gene Ontology
(Biological Process)
Complete annatation
actin filament-based movement [GO:0030048];
cellular response to insulin stimulus [GO:0032869];
chemical synaptic transmission [GO:0007268];
endoplasmic reticulum localization [GO:0051643];
exocytosis [GO:0006887];
insulin secretion [GO:0030073];
locomotion involved in locomotory behavior [GO:0031987];
long-chain fatty acid biosynthetic process [GO:0042759];
melanin biosynthetic process [GO:0042438];
melanocyte differentiation [GO:0030318];
melanosome transport [GO:0032402];
myelination [GO:0042552];
odontogenesis [GO:0042476];
post-Golgi vesicle-mediated transport [GO:0006892];
protein localization to plasma membrane [GO:0072659];
regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity [GO:0031585];
secretory granule localization [GO:0032252];
synapse organization [GO:0050808];
transport [GO:0006810];
vesicle-mediated transport [GO:0016192];
vesicle transport along actin filament [GO:0030050];
visual perception [GO:0007601]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
calcium ion binding [GO:0005509];
microfilament motor activity [GO:0000146];
poly(A RNA binding [GO:0044822];
Rab GTPase binding [GO:0017137]
Gene Ontology
(Cellular Component)
Complete annatation
actomyosin [GO:0042641];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
filopodium tip [GO:0032433];
Golgi apparatus [GO:0005794];
growth cone [GO:0030426];
insulin-responsive compartment [GO:0032593];
intermediate filament [GO:0005882];
melanosome [GO:0042470];
membrane [GO:0016020];
microtubule plus-end [GO:0035371];
myosin complex [GO:0016459];
neuronal cell body [GO:0043025];
neuron projection [GO:0043005];
photoreceptor outer segment [GO:0001750];
ruffle [GO:0001726]
Protein-protein interaction110728
Phylogenetic treeQ9Y4I1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6020397084960040.06769313608672780.127837482408114
AZA vs. DISU0.04361974654556710.8630300745519910.991133096247651
AZA vs. IL70.04674178707798850.8078809518267720.999311006273513
AZA vs. SAHA-0.549603014364950.02636603718804550.184061639502697
DISU vs. CD30.6327809656171630.08219144480451110.159865011481703
DISU vs. IL7-0.005822148482775630.9815505730649540.997754944136459
DISU vs. SAHA-0.5919266154253250.04450033031996170.261773801035185
DMSO vs. AZA-0.0005402302244632010.9974279524460731
DMSO vs. CD30.5913139661101520.06579865147291740.121239809740643
DMSO vs. DISU-0.04554981140458620.8518017724388330.982747872504457
DMSO vs. IL70.05440200726420480.7622135992833630.951841190064606
DMSO vs. SAHA-0.556123593814120.02536448183640310.165197971372701
HIV vs. Mock in Activation0.3647329183543360.5593857250426010.999983755607037
HIV vs. Mock in Latency-0.02020991881920240.9025373437237990.999834320637052
IL7 vs. CD30.6560398465282140.04245410776737780.0957724514821379
SAHA vs. CD30.02767770293448810.9376560035033720.957089946680832
SAHA vs. IL7-0.6000225120095250.01512096237283260.083492083079882
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.110116 0.534913
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4D07 X-ray 1.8Å B=1275-1297.
4J5L X-ray 2.2Å A/B=1448-1855.
4LLI X-ray 2.2Å A/B=1467-1855.
4LX1 X-ray 1.8Å A/B=1464-1855.
4LX2 X-ray 1.5Å A=1464-1855.
5JCY X-ray 1.8Å A=1464-1855.
5JCZ X-ray 2.0Å B/C/E=1464-1855.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found
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