Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001878
UniProt IDP35580
Primary gene name(s)MYH10
Synonym gene name(s)unknown
Protein nameMyosin-10
Protein functionCellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation, in the central part but not the margins of spreading cells, and lamellipodial extension; this function is mechanically antagonized by MYH9. {ECO:0000269|PubMed:20052411, ECO:0000269|PubMed:20603131}.
Subcellular locationCell projection, lamellipodium {ECO:0000269|PubMed:20052411, ECO:0000269|PubMed:22480440}. Note=Colocalizes with MCC at the leading edge of migrating cells.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P35580
Gene Ontology
(Biological Process)
Complete annatation
actin filament-based movement [GO:0030048];
actomyosin structure organization [GO:0031032];
adult heart development [GO:0007512];
aorta development [GO:0035904];
axon guidance [GO:0007411];
cardiac myofibril assembly [GO:0055003];
cardiac septum development [GO:0003279];
cell adhesion [GO:0007155];
cell proliferation [GO:0008283];
cerebellar Purkinje cell layer development [GO:0021680];
coronary vasculature development [GO:0060976];
exocytosis [GO:0006887];
fourth ventricle development [GO:0021592];
in utero embryonic development [GO:0001701];
lateral ventricle development [GO:0021670];
mitotic cytokinesis [GO:0000281];
neuromuscular process controlling balance [GO:0050885];
neuron migration [GO:0001764];
nuclear migration [GO:0007097];
plasma membrane repair [GO:0001778];
regulation of cell shape [GO:0008360];
retina development in camera-type eye [GO:0060041];
substrate-dependent cell migration, cell extension [GO:0006930];
third ventricle development [GO:0021678];
ventricular cardiac muscle cell development [GO:0055015]
Gene Ontology
(Molecular Function)
Complete annatation
actin binding [GO:0003779];
actin-dependent ATPase activity [GO:0030898];
actin filament binding [GO:0051015];
ADP binding [GO:0043531];
ATP binding [GO:0005524];
microfilament motor activity [GO:0000146]
Gene Ontology
(Cellular Component)
Complete annatation
actin cytoskeleton [GO:0015629];
actomyosin [GO:0042641];
axon [GO:0030424];
brush border [GO:0005903];
cell cortex [GO:0005938];
cleavage furrow [GO:0032154];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
dendritic spine [GO:0043197];
extracellular exosome [GO:0070062];
growth cone [GO:0030426];
lamellipodium [GO:0030027];
midbody [GO:0030496];
mitochondrion [GO:0005739];
myosin complex [GO:0016459];
myosin II complex [GO:0016460];
myosin II filament [GO:0097513];
neuromuscular junction [GO:0031594];
neuronal cell body [GO:0043025];
nucleus [GO:0005634];
plasma membrane [GO:0005886];
spindle [GO:0005819];
stress fiber [GO:0001725]
Protein-protein interaction110713
Phylogenetic treeP35580
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.005493395932160.004620381913076230.0135879797451483
AZA vs. DISU-0.242726478017920.3926158975359340.901952538630012
AZA vs. IL7-0.05425043533629030.8577615944049110.999311006273513
AZA vs. SAHA1.339772231448040.0001084223112398640.00399598513356898
DISU vs. CD3-1.260959456148110.0007541410279288120.00323687979925937
DISU vs. IL70.1803821429528510.5801542363278260.867125234249651
DISU vs. SAHA1.58373813259842.06933759772099e-050.00112996329475554
DMSO vs. AZA0.0008989085913381860.9973498886660391
DMSO vs. CD3-1.016548659049760.004992627545234880.013685213604251
DMSO vs. DISU0.2418597102105480.4085221892647140.870383340329923
DMSO vs. IL7-0.04777169954068820.8780368437791560.973265104409616
DMSO vs. SAHA1.331926640931370.0001702191669483710.00516171642873018
HIV vs. Mock in Activation-0.1139783122596180.8762544535627860.999983755607037
HIV vs. Mock in Latency0.002141332937818040.9901606390954270.999834320637052
IL7 vs. CD3-1.050236611716030.007985408242730970.0247764632446859
SAHA vs. CD30.3091609373024410.4786271142571510.5891456842159
SAHA vs. IL71.390218317798240.0003471554503639010.00519664474556647
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.204782 0.169721
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.02 0.972 1.064 1.14 1.034
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4PD3 X-ray 2.8Å A/B=1-782.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin cleaves 8424456
Pr55(Gag) interacts with 25010285
Tat interacts with 23732912

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04530 Tight junction - Homo sapiens (human)
hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human)
hsa05132 Salmonella infection - Homo sapiens (human)