Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001859
UniProt IDP11586
Primary gene name(s)MTHFD1
Synonym gene name(s)MTHFC, MTHFD
Protein nameC-1-tetrahydrofolate synthase, cytoplasmic
Protein functionunknown
Subcellular locationCytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P11586
Gene Ontology
(Biological Process)
Complete annatation
10-formyltetrahydrofolate biosynthetic process [GO:0009257];
embryonic neurocranium morphogenesis [GO:0048702];
embryonic viscerocranium morphogenesis [GO:0048703];
folic acid metabolic process [GO:0046655];
heart development [GO:0007507];
histidine biosynthetic process [GO:0000105];
methionine biosynthetic process [GO:0009086];
methionine metabolic process [GO:0006555];
neural tube closure [GO:0001843];
neutrophil homeostasis [GO:0001780];
one-carbon metabolic process [GO:0006730];
purine nucleotide biosynthetic process [GO:0006164];
serine family amino acid biosynthetic process [GO:0009070];
serine family amino acid metabolic process [GO:0009069];
somite development [GO:0061053];
tetrahydrofolate interconversion [GO:0035999];
transsulfuration [GO:0019346]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
formate-tetrahydrofolate ligase activity [GO:0004329];
methenyltetrahydrofolate cyclohydrolase activity [GO:0004477];
methylenetetrahydrofolate dehydrogenase, NAD+ activity [GO:0004487];
methylenetetrahydrofolate dehydrogenase, NADP+ activity [GO:0004488];
methylenetetrahydrofolate dehydrogenase [NAD(P+] activity [GO:0004486]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
membrane [GO:0016020];
mitochondrion [GO:0005739]
Protein-protein interaction110622
Phylogenetic treeP11586
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.237498923380020.003269037653879310.0101276332002608
AZA vs. DISU-0.3320998123176120.1905638492200420.778706964639598
AZA vs. IL70.6513416940832360.0007850031389434740.0661025268899161
AZA vs. SAHA-0.01707201474674710.9443073040635710.988712113735797
DISU vs. CD3-1.580895674975380.0003673850971612770.00175085771565257
DISU vs. IL70.9741505276538390.000137983575261180.00670147904923147
DISU vs. SAHA0.3164115294625120.2785152433033640.658741875678751
DMSO vs. AZA0.06437802594926250.7022304775526221
DMSO vs. CD3-1.183048727655910.004487051017772030.0125064613474071
DMSO vs. DISU0.395001666594320.1068203161664150.581051936518753
DMSO vs. IL70.5940479110779820.001033173229481040.0574726436694713
DMSO vs. SAHA-0.08820438008085980.7088075840424820.911894760281734
HIV vs. Mock in Activation0.04447611066080280.9603627508477040.999983755607037
HIV vs. Mock in Latency-0.006658223379397790.9680564343907310.999834320637052
IL7 vs. CD3-0.5788007863657470.1427589506155410.249365365194582
SAHA vs. CD3-1.278832945486710.002648623806748020.00825117227804551
SAHA vs. IL7-0.6710700848989410.006282052335369830.045352326103144
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.533473 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
0.016 0.003

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.009 0.931 0.977 0.926 0.978
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
202309_at 1.84 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00116 Tetrahydrofolic acid approved, nutraceutical unknown cofactor
DB00157 NADH nutraceutical unknown unknown
DB02358 LY374571 experimental unknown unknown
DB03461 2&,39;-Monophosphoadenosine 5&,39;-Diphosphoribose experimental unknown unknown
DB04322 LY249543 experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1A4I X-ray 1.5Å A/B=1-301.
1DIA X-ray 2.2Å A/B=1-306.
1DIB X-ray 2.7Å A/B=1-306.
1DIG X-ray 2.2Å A/B=1-306.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00670 One carbon pool by folate - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
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