Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001857
UniProt IDQ9Y483
Primary gene name(s)MTF2
Synonym gene name(s)PCL2
Protein nameMetal-response element-binding transcription factor 2
Protein functionPolycomb group, PcG that specifically binds histone H3 trimethylated at 'Lys-36', H3K36me3 and recruits the PRC2 complex. Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element, MRE of MT1A gene promoter, By similarity. {ECO:0000250}.
Subcellular locationNucleus {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y483
Gene Ontology
(Biological Process)
Complete annatation
covalent chromatin modification [GO:0016569];
negative regulation of gene expression, epigenetic [GO:0045814];
negative regulation of histone H3-K27 methylation [GO:0061086];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
positive regulation of histone H3-K27 methylation [GO:0061087];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
segment specification [GO:0007379];
stem cell differentiation [GO:0048863];
stem cell population maintenance [GO:0019827]
Gene Ontology
(Molecular Function)
Complete annatation
DNA binding [GO:0003677];
methylated histone binding [GO:0035064];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
ESC/E(Z complex [GO:0035098];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction116499
Phylogenetic treeQ9Y483
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.925357316051430.005150949727786620.0148697786434016
AZA vs. DISU0.0367338475749640.884838099681640.991861547789482
AZA vs. IL70.1364967225953850.4796015397488120.999311006273513
AZA vs. SAHA0.1975160440011020.4205832575823280.77445379597836
DISU vs. CD3-0.9017330488965440.01351455992639240.0373384304124705
DISU vs. IL70.09101263054088920.718336803993320.928295397381197
DISU vs. SAHA0.1618345430148360.5816783331666760.865212653639199
DMSO vs. AZA-0.05170169444121750.759155382080341
DMSO vs. CD3-0.988844308421890.00224591788710260.00689660680171233
DMSO vs. DISU-0.09026044068328480.712326965733090.962591472869635
DMSO vs. IL70.195384679235560.2796117907621380.771474402726639
DMSO vs. SAHA0.2423987883766850.3060999004489560.658844844282521
HIV vs. Mock in Activation-0.06202713267038060.9207220071956060.999983755607037
HIV vs. Mock in Latency-0.007330541477012190.9647595278922340.999834320637052
IL7 vs. CD3-0.7806307890063390.01575780316082030.0432400085641807
SAHA vs. CD3-0.7531217158208050.03579042278923420.0753508901136852
SAHA vs. IL70.05723249594459890.8153109309165950.920207202788459
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0664344 0.707288
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.992 1.01 1.017 1 1.132
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found