Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001853
UniProt IDQ13126
Primary gene name(s)MTAP
Synonym gene name(s)MSAP
Protein nameS-methyl-5'-thioadenosine phosphorylase
Protein functionCatalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine, MTA to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates. {ECO:0000255|HAMAP-Rule:MF_03155, ECO:0000269|PubMed:3091600}.
Subcellular locationCytoplasm. Nucleus {ECO:0000255|HAMAP-Rule:MF_03155}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13126
Gene Ontology
(Biological Process)
Complete annatation
L-methionine biosynthetic process from methylthioadenosine [GO:0019509];
nucleobase-containing compound metabolic process [GO:0006139];
purine-containing compound salvage [GO:0043101];
purine ribonucleoside salvage [GO:0006166]
Gene Ontology
(Molecular Function)
Complete annatation
phosphorylase activity [GO:0004645];
S-methyl-5-thioadenosine phosphorylase activity [GO:0017061]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
nucleus [GO:0005634]
Protein-protein interaction110611
Phylogenetic treeQ13126
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.3921228309874620.3642744664262090.486160625137125
AZA vs. DISU-0.2000624786685210.431973266118290.915650781529304
AZA vs. IL70.4422633184690240.02290466978157510.4815091877513
AZA vs. SAHA0.05599680258753720.8196471516122130.955293062273918
DISU vs. CD3-0.603361356779520.1584143399235390.266719285156644
DISU vs. IL70.633735429916750.01261581962332570.135458319673529
DISU vs. SAHA0.2561085958186160.3817177893209440.748584099265575
DMSO vs. AZA-0.01321992503146280.938086178984221
DMSO vs. CD3-0.4168657502973730.3257313103348480.439275104305298
DMSO vs. DISU0.1849020901294850.4514112308939920.888532369154407
DMSO vs. IL70.4628288315748060.01095287773151140.218127272991139
DMSO vs. SAHA0.0616503290130230.7950034757798150.94227063621473
HIV vs. Mock in Activation-0.2172129016664570.8221408172722010.999983755607037
HIV vs. Mock in Latency-0.1607141074085260.3370409284897130.999834320637052
IL7 vs. CD30.059800214533240.891132128905880.930876957655573
SAHA vs. CD3-0.3614281787893320.3956686237726170.511965118614421
SAHA vs. IL7-0.3906363308648240.1108768831967780.306277833481442
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0960585 0.598446
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB02158 (1s)-1-(9-Deazaadenin-9-Yl)-1,4,5-Trideoxy-1,4-Imino-5-Methylthio-D-Ribitol experimental unknown unknown
DB02281 Formycin experimental unknown unknown
DB02282 5&,39;-Deoxy-5&,39;-Methylthioadenosine experimental unknown unknown
DB00173 Adenine approved, nutraceutical unknown unknown
DB02933 5&,39;-Deoxy-5&,39;-(Methylthio)-Tubercidin experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1CB0 X-ray 1.7Å A=1-283.
1CG6 X-ray 1.7Å A=1-283.
1K27 X-ray 1.9Å A=1-283.
1SD1 X-ray 2.0Å A=1-283.
1SD2 X-ray 2.1Å A=1-283.
3LN5 X-ray 1.9Å C=227-237.
3OZC X-ray 1.9Å A=1-283.
3OZD X-ray 2.1Å A/B=1-283.
3OZE X-ray 2.0Å A/B/C/D/E/F=1-283.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00270 Cysteine and methionine metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)