Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001847
UniProt IDP20585
Primary gene name(s)MSH3
Synonym gene name(s)DUC1, DUG
Protein nameDNA mismatch repair protein Msh3
Protein functionComponent of the post-replicative DNA mismatch repair system, MMR. Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops, IDL up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P20585
Gene Ontology
(Biological Process)
Complete annatation
DNA repair [GO:0006281];
maintenance of DNA repeat elements [GO:0043570];
meiotic mismatch repair [GO:0000710];
mismatch repair [GO:0006298];
negative regulation of DNA recombination [GO:0045910];
positive regulation of helicase activity [GO:0051096];
reciprocal meiotic recombination [GO:0007131]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
double-strand/single-strand DNA junction binding [GO:0000406];
enzyme binding [GO:0019899];
heteroduplex DNA loop binding [GO:0000404];
Y-form DNA binding [GO:0000403]
Gene Ontology
(Cellular Component)
Complete annatation
membrane [GO:0016020];
MutSbeta complex [GO:0032302];
nuclear chromosome [GO:0000228];
nucleoplasm [GO:0005654]
Protein-protein interaction110574
Phylogenetic treeP20585
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.03720482382262030.9101847478424290.941850481415436
AZA vs. DISU-0.1330714389983540.601100225695740.955158276076142
AZA vs. IL70.123673726953390.5245048440188940.999311006273513
AZA vs. SAHA-0.5544432604538590.02602184774838610.182528307565925
DISU vs. CD3-0.1069740837622660.7701433732494460.840815615873263
DISU vs. IL70.2472113579239950.330028272426210.710333553230313
DISU vs. SAHA-0.4206203448905680.1578142795984540.512038537745598
DMSO vs. AZA-0.1533231487666530.3678329891615961
DMSO vs. CD3-0.1231213584003080.7024780112722210.781954360618564
DMSO vs. DISU-0.02104022805933550.9317022693909320.991044421109467
DMSO vs. IL70.2835115315367620.1195159175971020.610726150261665
DMSO vs. SAHA-0.4090457474979720.0892064683373780.34856918413461
HIV vs. Mock in Activation0.01628000686894630.9792071711034850.999983755607037
HIV vs. Mock in Latency0.1738770289447360.3003324715183970.999834320637052
IL7 vs. CD30.168593928931670.6012354133158540.717986165814441
SAHA vs. CD3-0.5421064023705640.1272592717720450.211366190109334
SAHA vs. IL7-0.6817047461470810.005919239114208770.0435464179636813
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.405639 0.600083
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.122 1.023 0.985 0.948 1.087
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3THW X-ray 3.0Å B=219-1134.
3THX X-ray 2.7Å B=219-1134.
3THY X-ray 2.8Å B=219-1134.
3THZ X-ray 4.3Å B=219-1134.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa01524 Platinum drug resistance - Homo sapiens (human)
hsa03430 Mismatch repair - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05210 Colorectal cancer - Homo sapiens (human)