Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001803
UniProt IDQ53F39
Primary gene name(s)MPPE1
Synonym gene name(s)PGAP5
Protein nameMetallophosphoesterase 1
Protein functionMetallophosphoesterase required for transport of GPI-anchor proteins from the endoplasmic reticulum to the Golgi. Acts in lipid remodeling steps of GPI-anchor maturation by mediating the removal of a side-chain ethanolamine-phosphate, EtNP from the second Man, Man2 of the GPI intermediate, an essential step for efficient transport of GPI-anchor proteins. {ECO:0000269|PubMed:19837036}.
Subcellular locationEndoplasmic reticulum-Golgi intermediate compartment membrane {ECO:0000269|PubMed:19837036};
Multi-pass membrane protein {ECO:0000269|PubMed:19837036}. Golgi apparatus, cis-Golgi network membrane {ECO:0000269|PubMed:19837036};
Multi-pass membrane protein {ECO:0000269|PubMed:19837036}. Note=Also localizes to endoplasmic reticulum exit site.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q53F39
Gene Ontology
(Biological Process)
Complete annatation
ER to Golgi vesicle-mediated transport [GO:0006888];
GPI anchor biosynthetic process [GO:0006506]
Gene Ontology
(Molecular Function)
Complete annatation
GPI anchor binding [GO:0034235];
manganese ion binding [GO:0030145];
phosphoric diester hydrolase activity [GO:0008081]
Gene Ontology
(Cellular Component)
Complete annatation
cis-Golgi network [GO:0005801];
endoplasmic reticulum exit site [GO:0070971];
endoplasmic reticulum-Golgi intermediate compartment [GO:0005793];
endoplasmic reticulum-Golgi intermediate compartment membrane [GO:0033116];
Golgi apparatus [GO:0005794];
integral component of membrane [GO:0016021];
nucleoplasm [GO:0005654]
Protein-protein interaction122415
Phylogenetic treeQ53F39
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.222425534658870.0009311282247584930.0034112699571123
AZA vs. DISU-0.1444092765067010.570859559205080.954304256505839
AZA vs. IL7-0.1683055544057690.3881650557674920.999311006273513
AZA vs. SAHA-0.7783115056694030.001777479200208990.0307267447981081
DISU vs. CD31.065950766186630.004496173608182890.0148402279329426
DISU vs. IL7-0.03377008384180380.8940969240878560.98053444331394
DISU vs. SAHA-0.6318573416398460.03237997506737960.216024171863868
DMSO vs. AZA0.04351735454931910.798586383752391
DMSO vs. CD31.25447796245170.0006171396928508210.00224299384144695
DMSO vs. DISU0.1861477677096760.4488010527811040.888470621945532
DMSO vs. IL7-0.204661038964920.2620792812835320.763448341130288
DMSO vs. SAHA-0.8269311912880570.0005979277307360810.0123443408629089
HIV vs. Mock in Activation0.03702823716451130.9641491530337960.999983755607037
HIV vs. Mock in Latency0.1747720197275350.2970442716098760.999834320637052
IL7 vs. CD31.059960690952270.003878970759052750.0135593057168836
SAHA vs. CD30.4214124219390040.2898407092064280.40320312819541
SAHA vs. IL7-0.6122515576846570.01352465392695150.0772822719085922
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.184487 0.730065
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found