Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001802
UniProt IDQ8N3R9
Primary gene name(s)MPP5
Synonym gene name(s)unknown
Protein nameMAGUK p55 subfamily member 5
Protein functionMay play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton, By similarity. {ECO:0000250}.
Subcellular locationCell membrane;
Peripheral membrane protein. Endomembrane system;
Peripheral membrane protein. Cell junction, tight junction. Note=Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membrane, OLM, apical to the adherens junction, AJ, where it colocalizes with CRB1. Colocalizes with MPP1 in the retina at the outer limiting membrane, OLM. Localized to the Purkinje cell body and axon.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8N3R9
Gene Ontology
(Biological Process)
Complete annatation
bicellular tight junction assembly [GO:0070830];
establishment of epithelial cell polarity [GO:0090162];
establishment of protein localization to plasma membrane [GO:0090002];
morphogenesis of an epithelial sheet [GO:0002011];
myelin assembly [GO:0032288];
peripheral nervous system myelin maintenance [GO:0032287];
protein localization to myelin sheath abaxonal region [GO:0035750]
Gene Ontology
(Molecular Function)
Complete annatation
protein domain specific binding [GO:0019904]
Gene Ontology
(Cellular Component)
Complete annatation
bicellular tight junction [GO:0005923];
cytoplasm [GO:0005737];
endomembrane system [GO:0012505];
extracellular exosome [GO:0070062];
lateral loop [GO:0043219];
myelin sheath adaxonal region [GO:0035749];
nucleoplasm [GO:0005654];
plasma membrane [GO:0005886];
protein complex [GO:0043234];
Schmidt-Lanterman incisure [GO:0043220]
Protein-protein interaction122155
Phylogenetic treeQ8N3R9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6711793901351890.04384648848022830.0894739066306528
AZA vs. DISU0.2446386054647610.3403992641723090.880262543573058
AZA vs. IL7-0.01748205676538690.9293668939622750.999311006273513
AZA vs. SAHA0.4507771976078910.06930746989691190.32443408445983
DISU vs. CD30.9037276564482070.01391081752272930.0382509418711457
DISU vs. IL7-0.2701214706590220.2899714194764420.676151453767573
DISU vs. SAHA0.2062817876933670.4856077878966990.815351489682615
DMSO vs. AZA-0.1415741033439410.4152133184425311
DMSO vs. CD30.5187177369757670.1104369232651970.185101500048559
DMSO vs. DISU-0.3877554961154780.1179402533812320.59626181709336
DMSO vs. IL70.1310521219479890.4785978415944820.880507468120249
DMSO vs. SAHA0.5839397914733550.01497542341749180.116638039444454
HIV vs. Mock in Activation-0.1386098498712370.8255558927213020.999983755607037
HIV vs. Mock in Latency-0.1013773187612720.550836429099970.999834320637052
IL7 vs. CD30.6640177110886470.04186183252081630.0947675277829624
SAHA vs. CD31.096177623033120.002553965098707530.00799506731149201
SAHA vs. IL70.4629920808753350.06161475418234640.210701374199887
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.24431 0.133759
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1Y76 NMR - B/D=118-177.
3UIT X-ray 2.0Å A/B/C/D=119-232.
4UU5 X-ray 1.2Å A=251-335.
4UU6 X-ray 1.8Å A=251-335.
4WSI X-ray 2.9Å A/B=236-675.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04390 Hippo signaling pathway - Homo sapiens (human)
hsa04530 Tight junction - Homo sapiens (human)