Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001801
UniProt IDQ99549
Primary gene name(s)MPHOSPH8
Synonym gene name(s)MPP8
Protein nameM-phase phosphoprotein 8
Protein functionHeterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3, H3K9me and promotes recruitment of proteins that mediate epigenetic repression, PubMed:20871592, PubMed:26022416. Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, PubMed:26022416. Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene, PubMed:20871592. Mediates down-regulation of CDH1 expression, PubMed:20871592. {ECO:0000269|PubMed:20871592, ECO:0000269|PubMed:26022416}.
Subcellular locationNucleus {ECO:0000269|PubMed:20871592, ECO:0000269|PubMed:23416073, ECO:0000269|PubMed:26022416, ECO:0000269|PubMed:8885239}. Chromosome {ECO:0000269|PubMed:23416073, ECO:0000269|PubMed:26022416}. Note=Detected on heterochromatin, PubMed:20871592, PubMed:26022416. Dissociates from chromatin during interphase and early mitosis, PubMed:23416073. Detected on nucleosomes, PubMed:20871592. {ECO:0000269|PubMed:20871592, ECO:0000269|PubMed:23416073, ECO:0000269|PubMed:26022416}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99549
Gene Ontology
(Biological Process)
Complete annatation
negative regulation of transcription, DNA-templated [GO:0045892];
regulation of DNA methylation [GO:0044030];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
methylated histone binding [GO:0035064]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
nuclear heterochromatin [GO:0005720];
nuclear nucleosome [GO:0000788];
nucleolus [GO:0005730];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction120119
Phylogenetic treeQ99549
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.08562839604901830.7934487960090260.859076000417596
AZA vs. DISU0.208197353875330.4124519706209740.909283245635738
AZA vs. IL7-0.2435030191133150.2050600481464120.967711366847485
AZA vs. SAHA-0.0453356353718030.8524761099482230.965456414827912
DISU vs. CD30.280586662177410.4420339178871070.573426788143739
DISU vs. IL7-0.4606684279448150.06925235228992940.349390632788048
DISU vs. SAHA-0.252132657665510.391144382518530.755664272392868
DMSO vs. AZA-0.06006862526763310.7195303200098441
DMSO vs. CD30.01324674185489430.9669093540057630.977288790211125
DMSO vs. DISU-0.2700835291614310.2708636783961820.790315589991932
DMSO vs. IL7-0.1762902055284120.3264910039955960.801757879084938
DMSO vs. SAHA0.008264234833128840.9720115314058870.993434544270861
HIV vs. Mock in Activation-0.01094087705686960.9859806303626870.999983755607037
HIV vs. Mock in Latency0.05331038125554270.7461142819475530.999834320637052
IL7 vs. CD3-0.1503715884408540.6393689596094250.74875365769504
SAHA vs. CD30.01533956962624330.9653192006968580.976791805177039
SAHA vs. IL70.1944906275206730.4243931096991280.666393600065979
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.00828585 0.976506
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.966 0.916 0.804 0.713 0.871
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3LWE X-ray 2.0Å A/B=55-116.
3QO2 X-ray 2.4Å A/B/C/D=55-116.
3R93 X-ray 2.0Å A/B/C/D=55-116.
3SVM X-ray 2.3Å A=55-116.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found