Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001789
UniProt IDP55196
Primary gene name(s)AFDN
Synonym gene name(s)AF6, MLLT4
Protein nameAfadin
Protein functionBelongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions, AJs. Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
Subcellular locationCell junction, adherens junction. Note=Not found at cell-matrix AJs.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P55196
Gene Ontology
(Biological Process)
Complete annatation
adherens junction organization [GO:0034332];
cell adhesion [GO:0007155];
cell-cell signaling [GO:0007267];
establishment of endothelial intestinal barrier [GO:0090557];
positive regulation of GTPase activity [GO:0043547];
regulation of protein localization [GO:0032880];
signal transduction [GO:0007165]
Gene Ontology
(Molecular Function)
Complete annatation
protein C-terminus binding [GO:0008022];
Ras GTPase binding [GO:0017016]
Gene Ontology
(Cellular Component)
Complete annatation
adherens junction [GO:0005912];
cell-cell junction [GO:0005911];
cell junction [GO:0030054];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleoplasm [GO:0005654];
plasma membrane [GO:0005886]
Protein-protein interaction110447
Phylogenetic treeP55196
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.01235435301244370.9698675355798440.979485626927057
AZA vs. DISU0.7478691028959240.003240782758715090.149104332055795
AZA vs. IL70.7108885670512980.002247724243196750.142352720662624
AZA vs. SAHA0.6344819749693030.1297797714190910.458966853568097
DISU vs. CD30.7226357520996690.046903855996090.102616650984769
DISU vs. IL7-0.04615783783259220.8543181149103430.970892912436268
DISU vs. SAHA-0.1107689680398750.7809287091422050.940053158220972
DMSO vs. AZA0.04273525260420780.8672662103672571
DMSO vs. CD30.02192501282808880.9452572985461960.962052506593598
DMSO vs. DISU-0.706031379270890.003929314891527810.134831771964225
DMSO vs. IL70.6749680455571760.003295640881229020.1119847770482
DMSO vs. SAHA0.5851459514324210.1623513858133840.487403431340129
HIV vs. Mock in Activation0.4844041420390950.4422426772307170.999983755607037
HIV vs. Mock in Latency0.3721804244353360.02398984478964780.586408938233195
IL7 vs. CD30.7046938149435630.02873896832698970.0701396485739502
SAHA vs. CD30.5985156749510770.1753849626318890.272901537105066
SAHA vs. IL7-0.07848044631603550.8452479514195970.934353366962412
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.126682 0.586874
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.076 1.08 1.161 1.22 1.188
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB08574 (5R)-2-SULFANYL-5-[4-(TRIFLUOROMETHYL)BENZYL]-1,3-THIAZOL-4-ONE experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1T2M NMR - A=1003-1094.
1XZ9 NMR - A=1001-1096.
2AIN NMR - A=1003-1094.
2EXG NMR - A=1001-1096.
5A6C X-ray 2.9Å A/B=1709-1746.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) incorporates 25631074
Tat upregulates 20139419

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04014 Ras signaling pathway - Homo sapiens (human)
hsa04015 Rap1 signaling pathway - Homo sapiens (human)
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04520 Adherens junction - Homo sapiens (human)
hsa04530 Tight junction - Homo sapiens (human)
hsa04670 Leukocyte transendothelial migration - Homo sapiens (human)