Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001783
UniProt IDQ9HBH9
Primary gene name(s)MKNK2
Synonym gene name(s)GPRK7, MNK2
Protein nameMAP kinase-interacting serine/threonine-protein kinase 2
Protein functionSerine/threonine-protein kinase that phosphorylates SFPQ/PSF, HNRNPA1 and EIF4E. May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Required for mediating PP2A-inhibition-induced EIF4E phosphorylation. Triggers EIF4E shuttling from cytoplasm to nucleus. Isoform 1 displays a high basal kinase activity, but isoform 2 exhibits a very low kinase activity. Acts as a mediator of the suppressive effects of IFNgamma on hematopoiesis. Negative regulator for signals that control generation of arsenic trioxide As(2O(3-dependent apoptosis and anti-leukemic responses. Involved in anti-apoptotic signaling in response to serum withdrawal. {ECO:0000269|PubMed:11154262, ECO:0000269|PubMed:11463832, ECO:0000269|PubMed:12897141, ECO:0000269|PubMed:16111636, ECO:0000269|PubMed:17965020, ECO:0000269|PubMed:18299328, ECO:0000269|PubMed:20823271, ECO:0000269|PubMed:20927323, ECO:0000269|PubMed:21149447}.
Subcellular locationIsoform 2: Nucleus, PML body.;
SUBCELLULAR LOCATION: Isoform 1: Cytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9HBH9
Gene Ontology
(Biological Process)
Complete annatation
cell surface receptor signaling pathway [GO:0007166];
cellular response to arsenic-containing substance [GO:0071243];
extrinsic apoptotic signaling pathway in absence of ligand [GO:0097192];
hemopoiesis [GO:0030097];
intracellular signal transduction [GO:0035556];
peptidyl-serine phosphorylation [GO:0018105];
protein autophosphorylation [GO:0046777];
protein phosphorylation [GO:0006468];
regulation of translation [GO:0006417]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
calcium-dependent protein serine/threonine kinase activity [GO:0009931];
calmodulin binding [GO:0005516];
calmodulin-dependent protein kinase activity [GO:0004683];
metal ion binding [GO:0046872];
protein serine/threonine kinase activity [GO:0004674]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
PML body [GO:0016605]
Protein-protein interaction109130
Phylogenetic treeQ9HBH9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.156280300652050.0005403363634272070.00212913716851631
AZA vs. DISU-0.1462992702760480.5622453152271630.951530917450798
AZA vs. IL7-0.06051827032256470.752628791156620.999311006273513
AZA vs. SAHA-0.1953519323611710.5162040409748720.831153569072337
DISU vs. CD30.9984884379840150.006714987328193420.0208146656354488
DISU vs. IL70.0758492360043650.7631591686748560.944226942383473
DISU vs. SAHA-0.04625609316116890.8888477089290980.97224755718618
DMSO vs. AZA-0.008325764625421470.9602319231698481
DMSO vs. CD31.13633638967840.0004782207708042830.00179437009020337
DMSO vs. DISU0.1361367319707780.5761395179262870.931580078313094
DMSO vs. IL7-0.04482970529358870.80266099717280.959941364004375
DMSO vs. SAHA-0.1916048239222680.4854559057536830.806340000641431
HIV vs. Mock in Activation0.5058644214544320.4177820795815550.999983755607037
HIV vs. Mock in Latency0.08751137061602120.5950346783647820.999834320637052
IL7 vs. CD31.100509565107710.0007199577115272190.00320778084122074
SAHA vs. CD30.9387492649503210.01151949132362030.0289427699644811
SAHA vs. IL7-0.1367613400005290.6538662489085660.831341079288776
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.4224 0.01878

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.382326 0.00532573
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2AC3 X-ray 2.1Å A=72-385.
2AC5 X-ray 3.2Å A=72-385.
2HW7 X-ray 2.7Å A=72-385.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr downregulates 16103188

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04010 MAPK signaling pathway - Homo sapiens (human)
hsa04066 HIF-1 signaling pathway - Homo sapiens (human)
hsa04910 Insulin signaling pathway - Homo sapiens (human)
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