Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001779
UniProt IDQ9NPJ1
Primary gene name(s)MKKS
Synonym gene name(s)BBS6
Protein nameMcKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin
Protein functionProbable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. May play a role in protein processing in limb, cardiac and reproductive system development. May play a role in cytokinesis. {ECO:0000269|PubMed:20080638}.
Subcellular locationCytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytosol. Note=The majority of the protein resides within the pericentriolar material, PCM, a proteinaceous tube surrounding centrioles. During interphase, the protein is confined to the lateral surfaces of the PCM but during mitosis it relocalizes throughout the PCM and is found at the intercellular bridge. The MKSS protein is highly mobile and rapidly shuttles between the cytosol and centrosome.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NPJ1
Gene Ontology
(Biological Process)
Complete annatation
artery smooth muscle contraction [GO:0014824];
brain morphogenesis [GO:0048854];
cartilage development [GO:0051216];
cerebral cortex development [GO:0021987];
chaperone-mediated protein complex assembly [GO:0051131];
cilium assembly [GO:0060271];
convergent extension involved in gastrulation [GO:0060027];
detection of mechanical stimulus involved in sensory perception of sound [GO:0050910];
determination of left/right symmetry [GO:0007368];
face development [GO:0060324];
fat cell differentiation [GO:0045444];
gonad development [GO:0008406];
heart development [GO:0007507];
heart looping [GO:0001947];
hippocampus development [GO:0021766];
intracellular transport [GO:0046907];
melanosome transport [GO:0032402];
negative regulation of actin filament polymerization [GO:0030837];
negative regulation of appetite by leptin-mediated signaling pathway [GO:0038108];
negative regulation of blood pressure [GO:0045776];
negative regulation of gene expression [GO:0010629];
negative regulation of GTPase activity [GO:0034260];
photoreceptor cell maintenance [GO:0045494];
pigment granule aggregation in cell center [GO:0051877];
positive regulation of multicellular organism growth [GO:0040018];
protein folding [GO:0006457];
regulation of cilium beat frequency involved in ciliary motility [GO:0060296];
regulation of stress fiber assembly [GO:0051492];
sensory perception of smell [GO:0007608];
social behavior [GO:0035176];
spermatid development [GO:0007286];
striatum development [GO:0021756];
vasodilation [GO:0042311];
visual perception [GO:0007601]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
RNA polymerase II repressing transcription factor binding [GO:0001103];
unfolded protein binding [GO:0051082]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
cytosol [GO:0005829];
intracellular [GO:0005622];
kinociliary basal body [GO:1902636];
motile cilium [GO:0031514]
Protein-protein interaction113837
Phylogenetic treeQ9NPJ1
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.2150368595214520.5132175966790090.628828194223724
AZA vs. DISU-0.09604770032194980.7050568031852730.971191581927376
AZA vs. IL70.1053563748513830.5860022201652840.999311006273513
AZA vs. SAHA-0.1828017065223710.455643128829670.795877163861952
DISU vs. CD30.1065203210378210.7698999087856650.840610692817261
DISU vs. IL70.1924653416151860.446124316531150.793909933609179
DISU vs. SAHA-0.08575686317575340.7691100543817250.934778841095724
DMSO vs. AZA-0.1101761254474230.514830119189981
DMSO vs. CD30.09294846645559990.7724450785841870.837849025046496
DMSO vs. DISU-0.01624371828648120.9470818184179910.993012243712903
DMSO vs. IL70.2228607781690430.2184180183353380.72717819563439
DMSO vs. SAHA-0.07945253705396480.7372360211519470.92359801120374
HIV vs. Mock in Activation-0.5012248011896690.4220732365051270.999983755607037
HIV vs. Mock in Latency-0.1384892946280760.4069486860105110.999834320637052
IL7 vs. CD30.3292423607832920.3081696551180530.446038142077435
SAHA vs. CD30.006953127945757410.9843440000715170.989399603851974
SAHA vs. IL7-0.2920817177531350.2324655914614520.472963466822774
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.187933 0.208893
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found