Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001774
UniProt IDQ8N4C8
Primary gene name(s)MINK1
Synonym gene name(s)B55, MAP4K6, MINK, YSK2, ZC3
Protein nameMisshapen-like kinase 1
Protein functionSerine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.; FUNCTION: Isoform 4 can activate the JNK pathway. Involved in the regulation of actin cytoskeleton reorganization, cell-matrix adhesion, cell-cell adhesion and cell migration.
Subcellular locationCytoplasm {ECO:0000269|PubMed:15469942, ECO:0000269|PubMed:18930710}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250}. Cell projection, axon {ECO:0000250}. Cell projection, dendrite {ECO:0000250}.;
SUBCELLULAR LOCATION: Isoform 4: Golgi apparatus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8N4C8
Gene Ontology
(Biological Process)
Complete annatation
actin cytoskeleton reorganization [GO:0031532];
chemical synaptic transmission [GO:0007268];
dendrite morphogenesis [GO:0048813];
intracellular signal transduction [GO:0035556];
JNK cascade [GO:0007254];
multicellular organism development [GO:0007275];
positive regulation of JNK cascade [GO:0046330];
protein autophosphorylation [GO:0046777];
protein phosphorylation [GO:0006468];
regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [GO:2000311];
regulation of cell-cell adhesion [GO:0022407];
regulation of cell-matrix adhesion [GO:0001952];
regulation of cell migration [GO:0030334];
response to stress [GO:0006950]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
protein kinase activity [GO:0004672];
protein serine/threonine kinase activity [GO:0004674];
receptor signaling protein serine/threonine kinase activity [GO:0004702]
Gene Ontology
(Cellular Component)
Complete annatation
axon [GO:0030424];
cell junction [GO:0030054];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
dendrite [GO:0030425];
extracellular exosome [GO:0070062];
Golgi apparatus [GO:0005794];
postsynaptic density [GO:0014069];
postsynaptic membrane [GO:0045211]
Protein-protein interaction119075
Phylogenetic treeQ8N4C8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.953256192857331.00276771330954e-081.18260680555566e-07
AZA vs. DISU0.2220216804828390.381062659867560.899651703517147
AZA vs. IL7-0.3380333192259810.07847219344257320.779800260380861
AZA vs. SAHA0.05030566457368850.8362747644434560.961440456451378
DISU vs. CD32.162247059793731.04356183694421e-081.60026969787355e-07
DISU vs. IL7-0.5693843803563230.02418445783882480.198141685826242
DISU vs. SAHA-0.1695945671775340.5614484624338090.857043020421981
DMSO vs. AZA0.1262223924493510.4498365010170521
DMSO vs. CD32.064940639600945.94959748134727e-108.01705599789773e-09
DMSO vs. DISU-0.09840044221122760.6867781780665790.957954410639349
DMSO vs. IL7-0.4565349665539090.01108199390837190.21981718180093
DMSO vs. SAHA-0.08099122452744330.7306914427441040.921387247641323
HIV vs. Mock in Activation0.4702026883663020.4512641712758910.999983755607037
HIV vs. Mock in Latency0.04264557064754810.7959604473263050.999834320637052
IL7 vs. CD31.621250953444839.00646235146851e-078.87864009311216e-06
SAHA vs. CD31.979747718173087.60685552325668e-088.28289831903744e-07
SAHA vs. IL70.3849074854019820.1140492851672320.311163606264407
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.375207 0.00605069
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found