Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001771
UniProt IDQ8N3F8
Primary gene name(s)MICALL1
Synonym gene name(s)KIAA1668, MIRAB13
Protein nameMICAL-like protein 1
Protein functionProbable lipid-binding protein with higher affinity for phosphatidic acid, a lipid enriched in recycling endosome membranes. On endosome membranes, may act as a downstream effector of Rab proteins recruiting cytosolic proteins to regulate membrane tubulation. May be involved in a late step of receptor-mediated endocytosis regulating for instance endocytosed-EGF receptor trafficking. Alternatively, may regulate slow endocytic recycling of endocytosed proteins back to the plasma membrane. May indirectly play a role in neurite outgrowth. {ECO:0000269|PubMed:19864458, ECO:0000269|PubMed:20801876, ECO:0000269|PubMed:21795389, ECO:0000269|PubMed:23596323}.
Subcellular locationRecycling endosome membrane;
Peripheral membrane protein. Late endosome membrane. Note=Localization to late endosomes is actin-dependent. Association to tubular recycling endosomes is regulated by RAB35 and ARF6.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q8N3F8
Gene Ontology
(Biological Process)
Complete annatation
cellular response to nerve growth factor stimulus [GO:1990090];
endocytosis [GO:0006897];
membrane tubulation [GO:0097320];
neuron projection development [GO:0031175];
protein localization to endosome [GO:0036010];
protein targeting to membrane [GO:0006612];
receptor-mediated endocytosis [GO:0006898];
retrograde transport, endosome to plasma membrane [GO:1990126];
slow endocytic recycling [GO:0032458]
Gene Ontology
(Molecular Function)
Complete annatation
identical protein binding [GO:0042802];
phosphatidic acid binding [GO:0070300];
Rab GTPase binding [GO:0017137];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
extrinsic component of membrane [GO:0019898];
late endosome [GO:0005770];
late endosome membrane [GO:0031902];
recycling endosome membrane [GO:0055038]
Protein-protein interaction124504
Phylogenetic treeQ8N3F8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6963943341014190.03460396237508250.0733442442578038
AZA vs. DISU-0.1438757442209590.5706781979664830.954304256505839
AZA vs. IL7-0.09891074951983350.6093297865187730.999311006273513
AZA vs. SAHA-1.162264367921360.00927577187066420.0930694526780756
DISU vs. CD30.5407948976526820.1372158096877050.239070220014795
DISU vs. IL70.03534183953106160.8887824162772570.979940654995064
DISU vs. SAHA-1.016108714622890.02820736611168320.199865169096933
DMSO vs. AZA0.01935496745545960.9087991289298021
DMSO vs. CD30.7040809072914360.02867564608907890.060504006769184
DMSO vs. DISU0.1612836692583590.5098918777533310.911919093829562
DMSO vs. IL7-0.110772475042690.5405841844761730.896696737481162
DMSO vs. SAHA-1.186937820782490.007195155913660710.0723118207517938
HIV vs. Mock in Activation0.2197016577705810.7253248771849710.999983755607037
HIV vs. Mock in Latency0.01162061239363890.9442230741472110.999834320637052
IL7 vs. CD30.6033500687933430.06174180442404920.130106438142526
SAHA vs. CD3-0.4890961253737890.2674425731412830.379064586793839
SAHA vs. IL7-1.065718591051930.01576820969548380.086275859509881
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0701045 0.693755
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.966 0.89 0.953 1.127 1.029
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2KSP NMR - B=419-433.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found