Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001770
UniProt IDQ7RTP6
Primary gene name(s)MICAL3
Synonym gene name(s)KIAA0819, KIAA1364
Protein name[F-actin]-methionine sulfoxide oxidase MICAL3
Protein functionMonooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization. Involved in exocytic vesicles tethering and fusion: the monooxygenase activity is required for this process. {ECO:0000269|PubMed:21596566, ECO:0000269|PubMed:24440334}.
Subcellular locationCytoplasm. Cytoplasm, cytoskeleton. Nucleus. Note=Mainly localizes in the nucleus. {ECO:0000269|PubMed:24440334}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q7RTP6
Gene Ontology
(Biological Process)
Complete annatation
actin filament depolymerization [GO:0030042];
cytoskeleton organization [GO:0007010];
exocytosis [GO:0006887];
oxidation-reduction process [GO:0055114]
Gene Ontology
(Molecular Function)
Complete annatation
actin binding [GO:0003779];
FAD binding [GO:0071949];
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(PH as one donor, and incorporation of one atom of oxygen [GO:0016709];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytoskeleton [GO:0005856];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction121609
Phylogenetic treeQ7RTP6
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.650915341066340.04957263274077460.0991584234464439
AZA vs. DISU0.1262706038000520.6198836151363720.959848547977984
AZA vs. IL70.116016238345440.551143804100790.999311006273513
AZA vs. SAHA0.3922416081337810.111466287415040.425665385841723
DISU vs. CD3-0.5374415587868780.1410938907187290.244240848494961
DISU vs. IL7-0.02020101021603450.9364515021896930.988381037522213
DISU vs. SAHA0.2688640052299830.3583386322661720.734744671289963
DMSO vs. AZA0.1299259099015470.4460299017432131
DMSO vs. CD3-0.5308521361541720.1019447219240560.173620610873704
DMSO vs. DISU0.002086145939772120.9932331913443510.998391502308472
DMSO vs. IL7-0.006584964889182270.9711305004679070.99414588445971
DMSO vs. SAHA0.2566745590682550.2817256554557820.631264845877801
HIV vs. Mock in Activation0.7221657896144360.2486004925016110.999983755607037
HIV vs. Mock in Latency0.1358981929421390.4152209280984780.999834320637052
IL7 vs. CD3-0.5305327829491070.1031081159737650.194292865617272
SAHA vs. CD3-0.282255106261340.4254729400277560.53988610625935
SAHA vs. IL70.2741310308726950.2650132450283840.510975908871561
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.572224 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.964 1.032 0.786 0.852 0.922
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2D88 NMR - A=518-625.
5SZG X-ray 2.7Å A/B=1841-1990.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found