Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001768
UniProt IDQ29983
Primary gene name(s)MICA
Synonym gene name(s)PERB11.1
Protein nameMHC class I polypeptide-related sequence A
Protein functionSeems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. {ECO:0000269|PubMed:10426993, ECO:0000269|PubMed:11224526, ECO:0000269|PubMed:11491531, ECO:0000269|PubMed:11777960, ECO:0000269|PubMed:9497295}.
Subcellular locationCell membrane {ECO:0000269|PubMed:10363723, ECO:0000269|PubMed:18287244, ECO:0000269|PubMed:8901601};
Single-pass type I membrane protein {ECO:0000269|PubMed:10363723, ECO:0000269|PubMed:18287244, ECO:0000269|PubMed:8901601}. Cytoplasm {ECO:0000269|PubMed:10363723, ECO:0000269|PubMed:18287244, ECO:0000269|PubMed:8901601}. Note=Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum. {ECO:0000269|PubMed:10363723, ECO:0000269|PubMed:18287244, ECO:0000269|PubMed:8901601}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q29983
Gene Ontology
(Biological Process)
Complete annatation
antigen processing and presentation [GO:0019882];
cellular response to DNA damage stimulus [GO:0006974];
cytolysis [GO:0019835];
defense response to bacterium [GO:0042742];
defense response to virus [GO:0051607];
gamma-delta T cell activation [GO:0046629];
immune response to tumor cell [GO:0002418];
natural killer cell mediated cytotoxicity [GO:0042267];
negative regulation of natural killer cell activation [GO:0032815];
negative regulation of natural killer cell mediated cytotoxicity [GO:0045953];
regulation of immune response [GO:0050776];
response to heat [GO:0009408];
T cell mediated cytotoxicity [GO:0001913]
Gene Ontology
(Molecular Function)
Complete annatation
antigen binding [GO:0003823];
natural killer cell lectin-like receptor binding [GO:0046703]
Gene Ontology
(Cellular Component)
Complete annatation
cell surface [GO:0009986];
cytoplasm [GO:0005737];
extracellular space [GO:0005615];
integral component of plasma membrane [GO:0005887];
plasma membrane [GO:0005886]
Protein-protein interaction319735
Phylogenetic treeQ29983
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.127106309343070.0007654100585737790.0028725748015
AZA vs. DISU0.03051616855334750.9051895883997280.993971427278471
AZA vs. IL7-0.2855219950362060.1487105192576360.916051268814065
AZA vs. SAHA0.4092818874936160.100751212547850.400951749838685
DISU vs. CD31.144948261784390.001968856301200470.00739559927343531
DISU vs. IL7-0.3257414000764210.2022155125524230.579103125328224
DISU vs. SAHA0.3811715452485070.196681787389830.568181634438425
DMSO vs. AZA-0.03481988538191660.8407340200633711
DMSO vs. CD31.08016073309130.0009498266352855330.0032711203880657
DMSO vs. DISU-0.06725202569490660.7854243952697910.973114958962133
DMSO vs. IL7-0.2433513400838930.1885441105427270.699385449417225
DMSO vs. SAHA0.4385699552182510.06812775346073490.304043047795894
HIV vs. Mock in Activation0.005914372656048710.9925114995059840.999983755607037
HIV vs. Mock in Latency0.05637818292380460.7397881607717910.999834320637052
IL7 vs. CD30.8475867210243040.009641230458985570.0289371299697908
SAHA vs. CD31.512214143293843.72606466654268e-050.000200613771078236
SAHA vs. IL70.6918135436347840.005477223680274030.041220004870919
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.911823 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1B3J X-ray 3.0Å A=24-297.
1HYR X-ray 2.7Å C=24-297.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef downregulates 17170457

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04650 Natural killer cell mediated cytotoxicity - Homo sapiens (human)