Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001767
UniProt IDO60502
Primary gene name(s)MGEA5
Synonym gene name(s)HEXC, KIAA0679, MEA5
Protein nameProtein O-GlcNAcase
Protein functionIsoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc, in vitro, PubMed:11148210. Does not bind acetyl-CoA and does not have histone acetyltransferase activity, PubMed:24088714. {ECO:0000269|PubMed:11148210, ECO:0000269|PubMed:11788610, ECO:0000269|PubMed:20673219, ECO:0000269|PubMed:22365600, ECO:0000269|PubMed:24088714}.; FUNCTION: Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc, in vitro, but has about six times lower specific activity than isoform 1. {ECO:0000269|PubMed:20673219}.
Subcellular locationIsoform 3: Nucleus {ECO:0000269|PubMed:11341771}.;
SUBCELLULAR LOCATION: Isoform 1: Cytoplasm {ECO:0000269|PubMed:11148210, ECO:0000269|PubMed:11341771, ECO:0000269|PubMed:11788610}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O60502
Gene Ontology
(Biological Process)
Complete annatation
glycoprotein catabolic process [GO:0006516];
N-acetylglucosamine metabolic process [GO:0006044];
protein deglycosylation [GO:0006517]
Gene Ontology
(Molecular Function)
Complete annatation
beta-N-acetylglucosaminidase activity [GO:0016231];
hyalurononglucosaminidase activity [GO:0004415]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
membrane [GO:0016020];
nucleus [GO:0005634]
Protein-protein interaction115948
Phylogenetic treeO60502
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.4376952647007380.1821232197542280.283687594887942
AZA vs. DISU0.3145250485977010.2132792252449710.801738414299923
AZA vs. IL7-0.1124518920177450.5574209804388830.999311006273513
AZA vs. SAHA0.007630275731102960.9750130225514340.993735382146066
DISU vs. CD30.7386098721116650.04233748723611690.0944634817343436
DISU vs. IL7-0.4357876852050210.08352060263864220.382668562162364
DISU vs. SAHA-0.3057123441081560.2934915874696170.676332066476239
DMSO vs. AZA-0.02192732789775710.8954342843725971
DMSO vs. CD30.4048693657557750.206075980775220.306408867980526
DMSO vs. DISU-0.3381053664309860.165415330783320.674146286131372
DMSO vs. IL7-0.08334335018664690.6419204503368710.922409998991473
DMSO vs. SAHA0.02218836306526340.92489672493360.982588646252719
HIV vs. Mock in Activation0.1336367021634070.8302108402467030.999983755607037
HIV vs. Mock in Latency0.08176168262411270.6188298651550330.999834320637052
IL7 vs. CD30.3331199402956990.2999659909361240.437906768172416
SAHA vs. CD30.4198237670814830.2346124716367020.341791559630189
SAHA vs. IL70.1157815719762920.6338871362674520.818854631253369
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-1.3 0.103605351 -1.5 0.023513763 -1.9 0.002970961
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.101084 0.533099
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.069 0.997 1.163 1.288 1.122
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
-0.53 0.0277 0.07 0.6556 0.2 0.0934 Protein Synthesis at 4; 8; and 20 hip
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2YDQ X-ray 2.6Å T=402-408.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04931 Insulin resistance - Homo sapiens (human)