Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001760
UniProt IDO95140
Primary gene name(s)MFN2
Synonym gene name(s)CPRP1, KIAA0214
Protein nameMitofusin-2
Protein functionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy, mitophagy. Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response, UPR upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions. {ECO:0000269|PubMed:11181170, ECO:0000269|PubMed:11950885, ECO:0000269|PubMed:15322553, ECO:0000269|PubMed:23620051, ECO:0000269|PubMed:23921378}.
Subcellular locationMitochondrion outer membrane {ECO:0000269|PubMed:11181170, ECO:0000269|PubMed:11950885, ECO:0000269|PubMed:12499352, ECO:0000269|PubMed:23620051};
Multi-pass membrane protein {ECO:0000269|PubMed:11181170, ECO:0000269|PubMed:11950885, ECO:0000269|PubMed:12499352, ECO:0000269|PubMed:23620051}. Note=Colocalizes with BAX during apoptosis.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O95140
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
blastocyst formation [GO:0001825];
blood coagulation [GO:0007596];
camera-type eye morphogenesis [GO:0048593];
macroautophagy [GO:0016236];
mitochondrial fusion [GO:0008053];
mitochondrial membrane organization [GO:0007006];
mitochondrion localization [GO:0051646];
negative regulation of Ras protein signal transduction [GO:0046580];
negative regulation of smooth muscle cell proliferation [GO:0048662];
parkin-mediated mitophagy in response to mitochondrial depolarization [GO:0061734];
protein localization to pre-autophagosomal structure [GO:0034497];
protein targeting to mitochondrion [GO:0006626];
response to unfolded protein [GO:0006986]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activity [GO:0003924];
GTP binding [GO:0005525];
ubiquitin protein ligase binding [GO:0031625]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
integral component of membrane [GO:0016021];
intrinsic component of mitochondrial outer membrane [GO:0031306];
microtubule cytoskeleton [GO:0015630];
mitochondrial outer membrane [GO:0005741];
mitochondrion [GO:0005739]
Protein-protein interaction115255
Phylogenetic treeO95140
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.236737792365980.0001963734933355670.000873337648732269
AZA vs. DISU0.1286307777187540.6112554050725950.957450739791279
AZA vs. IL70.1175569243993130.5409772621541930.999311006273513
AZA vs. SAHA-0.1892534020386710.4379749175998380.78651821633309
DISU vs. CD3-1.120576997186280.002312530420558350.00847684080713771
DISU vs. IL7-0.02071927662684370.93443011135270.988186499206029
DISU vs. SAHA-0.3154098452150690.2795392819126110.660215868673824
DMSO vs. AZA0.01684713423785050.9199650702521341
DMSO vs. CD3-1.230845626241880.000149682984634580.000643175745442086
DMSO vs. DISU-0.1135725714246770.6414929322351050.9472626202031
DMSO vs. IL70.1080150137840370.5480715404717590.897425363918391
DMSO vs. SAHA-0.2117202833332470.3693083466391240.717225659330031
HIV vs. Mock in Activation0.3282434920928370.5976669577014650.999983755607037
HIV vs. Mock in Latency0.1020783356877970.5367632348292430.999834320637052
IL7 vs. CD3-1.113588355940090.0006167116190252120.00280667096424125
SAHA vs. CD3-1.449316973067655.74768083400068e-050.000293618814130136
SAHA vs. IL7-0.3089509612077910.2048293355184440.438682311003887
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.203385 0.139967
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.816 1.082 0.873 0.915 1.11
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr downregulates 22438978

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04621 NOD-like receptor signaling pathway - Homo sapiens (human)