Protein Information (annotations from UniProt)
| Database ID | HIV0001760 |
| UniProt ID | O95140 |
| Primary gene name(s) | MFN2 |
| Synonym gene name(s) | CPRP1, KIAA0214 |
| Protein name | Mitofusin-2 |
| Protein function | Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy, mitophagy. Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response, UPR upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions. {ECO:0000269|PubMed:11181170, ECO:0000269|PubMed:11950885, ECO:0000269|PubMed:15322553, ECO:0000269|PubMed:23620051, ECO:0000269|PubMed:23921378}. |
| Subcellular location | Mitochondrion outer membrane {ECO:0000269|PubMed:11181170, ECO:0000269|PubMed:11950885, ECO:0000269|PubMed:12499352, ECO:0000269|PubMed:23620051}; Multi-pass membrane protein {ECO:0000269|PubMed:11181170, ECO:0000269|PubMed:11950885, ECO:0000269|PubMed:12499352, ECO:0000269|PubMed:23620051}. Note=Colocalizes with BAX during apoptosis. |
| ECO code | Click here for more information. |
| Amino acid sequence FASTA format: O95140 |
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| Gene Ontology (Biological Process) Complete annatation | apoptotic process [GO:0006915]; blastocyst formation [GO:0001825]; blood coagulation [GO:0007596]; camera-type eye morphogenesis [GO:0048593]; macroautophagy [GO:0016236]; mitochondrial fusion [GO:0008053]; mitochondrial membrane organization [GO:0007006]; mitochondrion localization [GO:0051646]; negative regulation of Ras protein signal transduction [GO:0046580]; negative regulation of smooth muscle cell proliferation [GO:0048662]; parkin-mediated mitophagy in response to mitochondrial depolarization [GO:0061734]; protein localization to pre-autophagosomal structure [GO:0034497]; protein targeting to mitochondrion [GO:0006626]; response to unfolded protein [GO:0006986] |
| Gene Ontology (Molecular Function) Complete annatation | GTPase activity [GO:0003924]; GTP binding [GO:0005525]; ubiquitin protein ligase binding [GO:0031625] |
| Gene Ontology (Cellular Component) Complete annatation | cytosol [GO:0005829]; integral component of membrane [GO:0016021]; intrinsic component of mitochondrial outer membrane [GO:0031306]; microtubule cytoskeleton [GO:0015630]; mitochondrial outer membrane [GO:0005741]; mitochondrion [GO:0005739] |
| Protein-protein interaction | 115255 |
| Phylogenetic tree | O95140 |
| HIV replication factor status |
Zhou et al., Cell. Host. Microbe., 2008 unknown Brass et al., Science, 2008 unknown Smith et al., J. Immunol, 2010 unknown |
| Interferon-stimulated gene status |
Lu et al., J. Virol., 2011 Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown; Schoggins JW and Rice CM, Curr. Opin. Virol., 2011 Targeted viruses: unknown Viral life cycle: unknown Mechanism related to antiviral activity: unknown |
| Anti-viral restriction factor |
Liu et al., Retrovirology, 2011 unknown (Triplicates) |
Gene Expression Profile top
Up-regulated; 
Down-regulated
For brief introduction to each study, please go to the help page.
Gene expression during HIV latency
| (1). Mohammadi et al., PLoS Pathog., 2014 Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model DMSO: Dimethyl suloxyde (negative control) - 0.0033% final SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies IL7: Interleukin-7 based stimulation DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM |
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| Experimental Condition | Log2 Fold Change | P value | Adjusted P value |
| AZA vs. CD3 | 1.23673779236598 | 0.000196373493335567 | 0.000873337648732269 |
| AZA vs. DISU | 0.128630777718754 | 0.611255405072595 | 0.957450739791279 |
| AZA vs. IL7 | 0.117556924399313 | 0.540977262154193 | 0.999311006273513 |
| AZA vs. SAHA | -0.189253402038671 | 0.437974917599838 | 0.78651821633309 |
| DISU vs. CD3 | -1.12057699718628 | 0.00231253042055835 | 0.00847684080713771 |
| DISU vs. IL7 | -0.0207192766268437 | 0.9344301113527 | 0.988186499206029 |
| DISU vs. SAHA | -0.315409845215069 | 0.279539281912611 | 0.660215868673824 |
| DMSO vs. AZA | 0.0168471342378505 | 0.919965070252134 | 1 |
| DMSO vs. CD3 | -1.23084562624188 | 0.00014968298463458 | 0.000643175745442086 |
| DMSO vs. DISU | -0.113572571424677 | 0.641492932235105 | 0.9472626202031 |
| DMSO vs. IL7 | 0.108015013784037 | 0.548071540471759 | 0.897425363918391 |
| DMSO vs. SAHA | -0.211720283333247 | 0.369308346639124 | 0.717225659330031 |
| HIV vs. Mock in Activation | 0.328243492092837 | 0.597666957701465 | 0.999983755607037 |
| HIV vs. Mock in Latency | 0.102078335687797 | 0.536763234829243 | 0.999834320637052 |
| IL7 vs. CD3 | -1.11358835594009 | 0.000616711619025212 | 0.00280667096424125 |
| SAHA vs. CD3 | -1.44931697306765 | 5.74768083400068e-05 | 0.000293618814130136 |
| SAHA vs. IL7 | -0.308950961207791 | 0.204829335518444 | 0.438682311003887 |
| (2). Iglesias-Ussel et al., J. Virol., 2013 Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model |
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| Log2 Fold Change | P Value | ||
| unknown | unknown | ||
Gene expression during HIV infection and replication
| (1). Imbeault et al., PloS Pathog., 2012 Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells |
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| Experiment type | Log2 Fold Change | P Value | Adjusted P Value | ||
| Infected vs. Mock | unknown | unknown | unknown | ||
| Infected vs. Bystander | unknown | unknown | unknown | ||
| (2). Lefebvre et al., J. Virol., 2011 Transcriptome analysis of T-cell line (Sup T1) |
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| Log2 Fold Change | unknown | ||||
| (3). Li et al., J. Immunol., 2013 Lymphatic tissue |
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| Acute Fold Change | Acute P Value | Asymt Fold Change | Asypt P Value | AIDS Fold Change | AIDS P Value |
| unknown | unknown | unknown | unknown | unknown | unknown |
| (4). Chang et al., MBio., 2011 Transcriptome analysis of T-cell line (Sup T1) Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation |
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| Up-regulated (True) | FALSE | ||||
| (5). Sherrill-Mix et al., BMC Retrovirol., 2015 Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based |
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| Test Status | Log2 Fold Change | P Value | |||
| OK | 0.203385 | 0.139967 | |||
| (6). Rotger et al., PLoS Pathog., 2010 Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient (Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach) |
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| Log2 Fold Change | P Value | ||||
| unknown | unknown | ||||
Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV
| (1). Greenwood et al., Elife, 2016 Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset |
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| 6 h | 24 h | 48 h | 72 h | RTi | ||
| 0.816 | 1.082 | 0.873 | 0.915 | 1.11 | ||
| (2). Navare et al., Virology, 2012 SUP-T1 cell line |
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| FC-4hpi | P-value | FC-8hpi | P-value | FC-20hpi | P-value | Category |
| unknown | unknown | unknown | unknown | unknown | unknown | unknown |
| (3). Hyrcza et al., J. Virolo., 2007 Primary human CD4+ and CD8+ T Cells |
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| Affymetrix Prob ID | Fold Change | In CD8? | Category | |||
| unknown | unknown | unknown | unknown | |||
Protein Overview top
Drug-protein Interaction (annotations from DrugBank) top
Protein Secondary Structure (annotations from PDB) top
HIV-1 Interaction (annotations from NCBI HIV-1 Interaction Database) top
| HIV Partner | Interaction Type | PubMed |
|---|---|---|
| Vpr | downregulates |
22438978 |
Metabolic/Signalling Pathway (annotations from KEGG database) top
| Pathway Accession Number | Description |
|---|---|
| hsa04621 | NOD-like receptor signaling pathway - Homo sapiens (human) |
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