Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001742
UniProt IDQ9H204
Primary gene name(s)MED28
Synonym gene name(s)EG1
Protein nameMediator of RNA polymerase II transcription subunit 28
Protein functionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways. {ECO:0000269|PubMed:15467741}.
Subcellular locationNucleus {ECO:0000269|PubMed:15467741}. Cytoplasm {ECO:0000269|PubMed:15467741}. Membrane {ECO:0000269|PubMed:15467741};
Peripheral membrane protein {ECO:0000269|PubMed:15467741}. Note=According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9H204
Gene Ontology
(Biological Process)
Complete annatation
negative regulation of smooth muscle cell differentiation [GO:0051151];
regulation of transcription, DNA-templated [GO:0006355];
stem cell population maintenance [GO:0019827];
transcription, DNA-templated [GO:0006351]
Gene Ontology
(Molecular Function)
Complete annatation
unknown
Gene Ontology
(Cellular Component)
Complete annatation
cortical actin cytoskeleton [GO:0030864];
mediator complex [GO:0016592];
membrane [GO:0016020]
Protein-protein interaction123219
Phylogenetic treeQ9H204
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is also observed
Brass et al., Science, 2008
      Yes
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.183313318531670.5760168091599990.68403130693819
AZA vs. DISU-0.006759269156874280.9786912358977790.997889048290254
AZA vs. IL7-0.05705807130036080.7670804794428090.999311006273513
AZA vs. SAHA-0.01101386708974470.964066240637750.991027794621028
DISU vs. CD3-0.202388988303570.5773545806565380.692311108807307
DISU vs. IL7-0.05972664674429390.812660994511930.959830555506168
DISU vs. SAHA-0.002814379632948830.9923087419927410.999572425883731
DMSO vs. AZA-0.07103778566559340.6724949032958131
DMSO vs. CD3-0.2640702017737390.4095197048332720.52374786466434
DMSO vs. DISU-0.06560014092619740.7881561229261160.973692001196281
DMSO vs. IL70.02095276614136450.9074390704998710.98028625138788
DMSO vs. SAHA0.05324004740552550.8216797297567840.951481767742181
HIV vs. Mock in Activation0.1506873143331050.8085908940682810.999983755607037
HIV vs. Mock in Latency0.1796717278290990.2775777157001740.999834320637052
IL7 vs. CD3-0.2336001820083660.4678492712154780.600081931441622
SAHA vs. CD3-0.218376081496090.5363281998493310.642077055269257
SAHA vs. IL70.04275809322673440.8609384438843480.942022560575218
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.450021 0.000580925
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.037 0.999 0.995 1.075 1.023
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat regulated by 18187620
18976975
HIV-1 virus replication enhanced by expression of human gene 18187620
2510071918976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found
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