Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001740
UniProt IDQ15648
Primary gene name(s)MED1
Synonym gene name(s)ARC205, CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2
Protein nameMediator of RNA polymerase II transcription subunit 1
Protein functionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors, PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells, PubMed:24245781. {ECO:0000269|PubMed:10406464, ECO:0000269|PubMed:11867769, ECO:0000269|PubMed:12037571, ECO:0000269|PubMed:12218053, ECO:0000269|PubMed:12556447, ECO:0000269|PubMed:14636573, ECO:0000269|PubMed:15340084, ECO:0000269|PubMed:15471764, ECO:0000269|PubMed:15989967, ECO:0000269|PubMed:16574658, ECO:0000269|PubMed:24245781, ECO:0000269|PubMed:9653119}.
Subcellular locationNucleus {ECO:0000269|PubMed:15471764, ECO:0000269|PubMed:16314496, ECO:0000269|PubMed:16574658}. Note=A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q15648
Gene Ontology
(Biological Process)
Complete annatation
androgen biosynthetic process [GO:0006702];
androgen receptor signaling pathway [GO:0030521];
angiogenesis [GO:0001525];
animal organ regeneration [GO:0031100];
brain development [GO:0007420];
cell morphogenesis [GO:0000902];
cellular lipid metabolic process [GO:0044255];
cellular response to epidermal growth factor stimulus [GO:0071364];
cellular response to hepatocyte growth factor stimulus [GO:0035729];
cellular response to steroid hormone stimulus [GO:0071383];
cellular response to thyroid hormone stimulus [GO:0097067];
embryonic heart tube development [GO:0035050];
embryonic hemopoiesis [GO:0035162];
embryonic hindlimb morphogenesis [GO:0035116];
embryonic placenta development [GO:0001892];
enucleate erythrocyte development [GO:0048822];
epithelial cell proliferation involved in mammary gland duct elongation [GO:0060750];
ERK1 and ERK2 cascade [GO:0070371];
erythrocyte development [GO:0048821];
fat cell differentiation [GO:0045444];
intracellular steroid hormone receptor signaling pathway [GO:0030518];
keratinocyte differentiation [GO:0030216];
lactation [GO:0007595];
lens development in camera-type eye [GO:0002088];
liver development [GO:0001889];
mammary gland branching involved in pregnancy [GO:0060745];
mammary gland branching involved in thelarche [GO:0060744];
megakaryocyte development [GO:0035855];
monocyte differentiation [GO:0030224];
mRNA transcription from RNA polymerase II promoter [GO:0042789];
negative regulation of apoptotic process [GO:0043066];
negative regulation of keratinocyte proliferation [GO:0010839];
negative regulation of neuron differentiation [GO:0045665];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
peroxisome proliferator activated receptor signaling pathway [GO:0035357];
positive regulation of erythrocyte differentiation [GO:0045648];
positive regulation of G0 to G1 transition [GO:0070318];
positive regulation of gene expression [GO:0010628];
positive regulation of hepatocyte proliferation [GO:2000347];
positive regulation of interferon-gamma-mediated signaling pathway [GO:0060335];
positive regulation of intracellular estrogen receptor signaling pathway [GO:0033148];
positive regulation of keratinocyte differentiation [GO:0045618];
positive regulation of mammary gland epithelial cell proliferation [GO:0033601];
positive regulation of protein import into nucleus, translocation [GO:0033160];
positive regulation of receptor activity [GO:2000273];
positive regulation of transcription, DNA-templated [GO:0045893];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
regulation of cell cycle [GO:0051726];
regulation of RNA biosynthetic process [GO:2001141];
regulation of transcription from RNA polymerase I promoter [GO:0006356];
regulation of vitamin D receptor signaling pathway [GO:0070562];
retinal pigment epithelium development [GO:0003406];
thyroid hormone generation [GO:0006590];
thyroid hormone mediated signaling pathway [GO:0002154];
transcription initiation from RNA polymerase II promoter [GO:0006367];
ventricular trabecula myocardium morphogenesis [GO:0003222]
Gene Ontology
(Molecular Function)
Complete annatation
chromatin binding [GO:0003682];
chromatin DNA binding [GO:0031490];
core promoter binding [GO:0001047];
estrogen receptor binding [GO:0030331];
LBD domain binding [GO:0050693];
ligand-dependent nuclear receptor binding [GO:0016922];
ligand-dependent nuclear receptor transcription coactivator activity [GO:0030374];
mediator complex binding [GO:0036033];
nuclear hormone receptor binding [GO:0035257];
peroxisome proliferator activated receptor binding [GO:0042975];
receptor activity [GO:0004872];
retinoic acid receptor binding [GO:0042974];
RNA polymerase II core promoter proximal region sequence-specific DNA binding [GO:0000978];
RNA polymerase II transcription cofactor activity [GO:0001104];
RNA polymerase II transcription factor activity, sequence-specific DNA binding [GO:0000981];
thyroid hormone receptor binding [GO:0046966];
thyroid hormone receptor coactivator activity [GO:0030375];
transcription coactivator activity [GO:0003713];
transcription cofactor activity [GO:0003712];
transcription factor binding [GO:0008134];
ubiquitin protein ligase activity [GO:0061630];
vitamin D receptor binding [GO:0042809]
Gene Ontology
(Cellular Component)
Complete annatation
chromatin [GO:0000785];
mediator complex [GO:0016592];
membrane [GO:0016020];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
protein-DNA complex [GO:0032993];
ubiquitin ligase complex [GO:0000151]
Protein-protein interaction111465
Phylogenetic treeQ15648
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5762241710451120.07994316703102010.146439403681519
AZA vs. DISU0.1551253723002180.5395980161835560.943295487427274
AZA vs. IL70.1981970420937290.3021571879212130.999311006273513
AZA vs. SAHA-0.3807139193035570.1188860249522590.438807037250332
DISU vs. CD3-0.433888226222310.2350434381180590.359736499765622
DISU vs. IL70.0340586379951050.8923379168140960.98029047000329
DISU vs. SAHA-0.5344369150487680.06714594987857760.327926103781239
DMSO vs. AZA-0.01517102272887930.9277690492904211
DMSO vs. CD3-0.6017124865534850.06146378604095230.114498817448615
DMSO vs. DISU-0.171834712650130.4810126551523920.901849833638261
DMSO vs. IL70.2205171548046910.2196353931289650.727735415657001
DMSO vs. SAHA-0.3725917467881620.1141906356053750.401633536261397
HIV vs. Mock in Activation0.4542009383765120.4691915689985940.999983755607037
HIV vs. Mock in Latency0.0825767975084570.6165786692884190.999834320637052
IL7 vs. CD3-0.3708499607129940.2505141382682020.382379706948518
SAHA vs. CD3-0.981457097380450.006081471408507570.0168622099135862
SAHA vs. IL7-0.5822832659224750.01704410063908050.0905086284385247
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.331788 0.0092834
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.001 0.937 0.824 0.875 0.921
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB08696 5-{2-[1-(1-METHYL-PROPYL)-7A-METHYL-OCTAHYDRO-INDEN-4-YLIDENE]-ETHYLIDENE}-2-METHYLENE-CYCLOHEXANE-1,3-DIOL experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1RJK X-ray 1.9Å C=640-652.
1RK3 X-ray 2.2Å C=640-652.
1RKG X-ray 1.9Å C=640-652.
1RKH X-ray 2.2Å C=640-652.
2O4J X-ray 1.7Å C=640-652.
2O4R X-ray 1.9Å C=640-652.
2ZFX X-ray 1.9Å C=640-652.
3A2H X-ray 2.5Å B=640-652.
3AUN X-ray 1.8Å B=640-652.
3VJS X-ray 1.9Å C=640-652.
3VJT X-ray 2.0Å C=640-652.
3VRT X-ray 2.4Å C=640-652.
3VRU X-ray 2.0Å C=640-652.
3VRV X-ray 1.9Å C=640-652.
3VRW X-ray 2.4Å C=640-652.
3W0G X-ray 1.9Å C=640-652.
3W0H X-ray 1.8Å C=640-652.
3W0I X-ray 1.9Å C=640-652.
3W0J X-ray 1.8Å C=640-652.
3W5P X-ray 1.9Å C=640-652.
3W5Q X-ray 1.9Å C=640-652.
3W5R X-ray 2.2Å C=640-652.
3W5T X-ray 2.2Å C=640-652.
3WT5 X-ray 1.9Å C=640-652.
3WT6 X-ray 2.0Å C=640-652.
3WT7 X-ray 2.4Å C=640-652.
3WTQ X-ray 2.1Å C=640-652.
4YNK X-ray 2.3Å C=640-652.
5AWJ X-ray 2.2Å C=640-652.
5AWK X-ray 2.9Å C=640-652.
5B5B X-ray 2.0Å C/F=640-652.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
matrix upregulates 26469385
26469385

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa01522 Endocrine resistance - Homo sapiens (human)
hsa04919 Thyroid hormone signaling pathway - Homo sapiens (human)
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