Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001739
UniProt IDQ9NVC6
Primary gene name(s)MED17
Synonym gene name(s)ARC77, CRSP6, DRIP77, DRIP80, TRAP80
Protein nameMediator of RNA polymerase II transcription subunit 17
Protein functionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. {ECO:0000269|PubMed:16595664}.
Subcellular locationNucleus {ECO:0000305}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NVC6
Gene Ontology
(Biological Process)
Complete annatation
androgen receptor signaling pathway [GO:0030521];
intracellular steroid hormone receptor signaling pathway [GO:0030518];
positive regulation of transcription, DNA-templated [GO:0045893];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
regulation of transcription from RNA polymerase II promoter [GO:0006357];
stem cell population maintenance [GO:0019827];
transcription initiation from RNA polymerase II promoter [GO:0006367]
Gene Ontology
(Molecular Function)
Complete annatation
ligand-dependent nuclear receptor transcription coactivator activity [GO:0030374];
receptor activity [GO:0004872];
RNA polymerase II transcription cofactor activity [GO:0001104];
thyroid hormone receptor binding [GO:0046966];
transcription coactivator activity [GO:0003713];
transcription cofactor activity [GO:0003712];
ubiquitin protein ligase activity [GO:0061630];
vitamin D receptor binding [GO:0042809]
Gene Ontology
(Cellular Component)
Complete annatation
mediator complex [GO:0016592];
membrane [GO:0016020];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
transcription factor complex [GO:0005667];
ubiquitin ligase complex [GO:0000151]
Protein-protein interaction114830
Phylogenetic treeQ9NVC6
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is also observed
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5315101201128520.1055457964685710.184012289690491
AZA vs. DISU0.5326315723784560.03688672944816420.444824447761301
AZA vs. IL70.05540425031773890.7744202226312470.999311006273513
AZA vs. SAHA-0.04932333567619250.8402903855321270.962210367024559
DISU vs. CD3-0.01153416601532330.974720981277880.984082791270247
DISU vs. IL7-0.4861894667502360.0555753492788270.309788752488253
DISU vs. SAHA-0.580522812226020.0483698232733920.274259445655866
DMSO vs. AZA-0.03283726171559110.8458584872855321
DMSO vs. CD3-0.5747296567672520.07321580298610010.132285448786951
DMSO vs. DISU-0.5669489415725680.02133494738304930.30249404445769
DMSO vs. IL70.09540474011375570.5978641046327180.911970258876874
DMSO vs. SAHA-0.02318758082925950.9218926474031780.981876243091941
HIV vs. Mock in Activation0.04275851757303620.9451910103769710.999983755607037
HIV vs. Mock in Latency-0.01567561897784530.9247553021996440.999834320637052
IL7 vs. CD3-0.4688036779747140.1453388455700750.252733320175097
SAHA vs. CD3-0.6053659093113250.08770537277569360.156731882809167
SAHA vs. IL7-0.1078645736700940.6587469580014410.834940859742177
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.174431 0.25895
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.958 1.02 0.906 1.003 1.037
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat regulated by 18976975
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04919 Thyroid hormone signaling pathway - Homo sapiens (human)