Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001737
UniProt IDP23368
Primary gene name(s)ME2
Synonym gene name(s)unknown
Protein nameNAD-dependent malic enzyme, mitochondrial
Protein functionunknown
Subcellular locationMitochondrion matrix.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P23368
Gene Ontology
(Biological Process)
Complete annatation
malate metabolic process [GO:0006108];
pyruvate metabolic process [GO:0006090];
regulation of NADP metabolic process [GO:1902031]
Gene Ontology
(Molecular Function)
Complete annatation
electron carrier activity [GO:0009055];
malate dehydrogenase, decarboxylating, NAD+ activity [GO:0004471];
malic enzyme activity [GO:0004470];
metal ion binding [GO:0046872];
NAD binding [GO:0051287];
oxaloacetate decarboxylase activity [GO:0008948]
Gene Ontology
(Cellular Component)
Complete annatation
intracellular membrane-bounded organelle [GO:0043231];
mitochondrial matrix [GO:0005759];
mitochondrion [GO:0005739]
Protein-protein interaction110364
Phylogenetic treeP23368
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.184842989186980.0003548752253171860.00146861048763883
AZA vs. DISU-0.233063207766460.3578544968265690.892482504096917
AZA vs. IL70.2898538159891870.1322674504346950.897911842036448
AZA vs. SAHA-0.163620451292330.5027767616953970.822347019592093
DISU vs. CD3-1.430289748342870.0001128163204895390.000631955858438988
DISU vs. IL70.5140853120941110.04220197141525830.271529969733256
DISU vs. SAHA0.07017158171184580.8098369684322280.950589679896226
DMSO vs. AZA-0.05181238367558360.7576376170274121
DMSO vs. CD3-1.247771482370040.0001200620125476610.000529024340166868
DMSO vs. DISU0.1795174875495430.4626765818925280.893512623312363
DMSO vs. IL70.3488370119287110.05287731570500440.451629197877021
DMSO vs. SAHA-0.1190867854097350.6137132960938620.872526096111373
HIV vs. Mock in Activation-0.07125653567854260.9087809810814160.999983755607037
HIV vs. Mock in Latency-0.08666546961004820.6002633958361810.999834320637052
IL7 vs. CD3-0.8863748401604040.006115702214250620.0198580972994631
SAHA vs. CD3-1.37361853986830.0001342744868880750.000621638289355495
SAHA vs. IL7-0.457054716528640.06118760880184760.209766207659552
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
2 9.18E-06 1.4 0.003694353 1.7 0.005074594
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.702527 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.032 1.014 1.115 1.094 0.944
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00157 NADH nutraceutical unknown unknown
DB01677 Fumarate experimental unknown unknown
DB03499 Malate Ion experimental unknown unknown
DB03589 Alpha-Ketomalonic Acid experimental unknown unknown
DB03680 Tartronate experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1DO8 X-ray 2.2Å A/B/C/D=21-584.
1EFK X-ray 2.6Å A/B/C/D=1-584.
1EFL X-ray 2.6Å A/B/C/D=1-584.
1GZ3 X-ray 2.3Å A/B/C/D=20-573.
1GZ4 X-ray 2.2Å A/B/C/D=23-573.
1PJ2 X-ray 2.3Å A/B/C/D=21-584.
1PJ3 X-ray 2.1Å A/B/C/D=21-584.
1PJ4 X-ray 2.3Å A/B/C/D=21-584.
1PJL X-ray 2.9Å A/B/C/D/E/F/G/H=1-584.
1QR6 X-ray 2.1Å A/B=1-584.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00620 Pyruvate metabolism - Homo sapiens (human)
hsa01200 Carbon metabolism - Homo sapiens (human)