Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001735
UniProt IDO15151
Primary gene name(s)MDM4
Synonym gene name(s)MDMX
Protein nameProtein Mdm4
Protein functionInhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. {ECO:0000269|PubMed:16163388, ECO:0000269|PubMed:16511572}.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O15151
Gene Ontology
(Biological Process)
Complete annatation
cell proliferation [GO:0008283];
cellular response to hypoxia [GO:0071456];
DNA damage response, signal transduction by p53 class mediator [GO:0030330];
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [GO:0006977];
G0 to G1 transition [GO:0045023];
negative regulation of apoptotic process [GO:0043066];
negative regulation of cell cycle arrest [GO:0071157];
negative regulation of cell proliferation [GO:0008285];
negative regulation of protein catabolic process [GO:0042177];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
protein complex assembly [GO:0006461];
protein stabilization [GO:0050821];
regulation of signal transduction by p53 class mediator [GO:1901796]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
ubiquitin protein ligase activity [GO:0061630];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction110359
Phylogenetic treeO15151
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.185746933248220.0003546747163843960.00146818383008668
AZA vs. DISU0.09140801048002630.7177275955295270.973374730783303
AZA vs. IL7-0.04523538188768570.8141414874597810.999311006273513
AZA vs. SAHA-0.2732923232213190.264671755427990.637085023192615
DISU vs. CD31.264462288077760.0005932500049254940.00263717209503005
DISU vs. IL7-0.1454668676227570.5637876780453220.858494873387195
DISU vs. SAHA-0.3640837709940320.2129760530102630.587473442131041
DMSO vs. AZA-0.009762865047718770.9535749228163061
DMSO vs. CD31.167099048802820.0003166902510990390.0012460012099261
DMSO vs. DISU-0.1021030984394840.6754828809041630.955152420929036
DMSO vs. IL7-0.02857924710944010.8738296736574340.972518772476859
DMSO vs. SAHA-0.271740588381930.2509994385417410.599573274110098
HIV vs. Mock in Activation-0.1173819587795970.8504767759284510.999983755607037
HIV vs. Mock in Latency0.1167663192471220.4793368678460380.999834320637052
IL7 vs. CD31.148687387787490.0004140898221443030.0019883962692517
SAHA vs. CD30.8864028877078050.01329847621334010.0325813748227988
SAHA vs. IL7-0.2332272418725810.3411469845745760.590746656574056
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-1.7 0.201615075 -2.1 0.046251933 -3 0.01929575
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0229661 0.941141
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2CR8 NMR - A=300-339.
2MWY NMR - A=23-111.
2N06 NMR - A=23-111.
2N0U NMR - A=23-111.
2N0W NMR - A=23-111.
2N14 NMR - A=23-111.
2VJE X-ray 2.2Å B/D=428-490.
2VJF X-ray 2.3Å B/D=428-490.
2VYR X-ray 2.0Å A/B/C/D=16-116.
3DAB X-ray 1.9Å A/C/E/G=23-111.
3EQY X-ray 1.6Å A/B=24-108.
3FDO X-ray 1.4Å A=23-111.
3FE7 X-ray 1.3Å A=14-111.
3FEA X-ray 1.3Å A=14-111.
3JZO X-ray 1.8Å A=23-111.
3JZP X-ray 1.7Å A=23-111.
3JZQ X-ray 1.8Å A/B=23-111.
3LBJ X-ray 1.5Å E=23-111.
3MQR X-ray 1.8Å B=395-404.
3U15 X-ray 1.8Å A/B/C/D=14-111.
4RXZ X-ray 1.5Å A/B=24-108.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04115 p53 signaling pathway - Homo sapiens (human)
hsa05206 MicroRNAs in cancer - Homo sapiens (human)