Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001732
UniProt IDQ9UJA3
Primary gene name(s)MCM8
Synonym gene name(s)C20orf154
Protein nameDNA helicase MCM8
Protein functionComponent of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex, pre-RC during G(1 phase by recruiting CDC6 to the origin recognition complex, ORC. Binds chromatin throughout the cell cycle. {ECO:0000269|PubMed:22771115}.
Subcellular locationNucleus {ECO:0000269|PubMed:12527764, ECO:0000269|PubMed:18072282}. Note=Localizes to nuclear foci and colocalizes with RAD51.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UJA3
Gene Ontology
(Biological Process)
Complete annatation
cellular response to DNA damage stimulus [GO:0006974];
DNA replication [GO:0006260];
double-strand break repair via homologous recombination [GO:0000724];
female gamete generation [GO:0007292];
G1/S transition of mitotic cell cycle [GO:0000082];
male gamete generation [GO:0048232]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
DNA binding [GO:0003677];
helicase activity [GO:0004386]
Gene Ontology
(Cellular Component)
Complete annatation
MCM8-MCM9 complex [GO:0097362];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction124109
Phylogenetic treeQ9UJA3
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.100561945230410.1652184966996030.263037201041134
AZA vs. DISU0.5289857060588570.1564305946273910.738732701570578
AZA vs. IL70.1745178815354940.5032163455953560.999311006273513
AZA vs. SAHA-0.3219739309112180.193334918701160.560125036421542
DISU vs. CD3-0.5852161419727350.46770813171870.596106710772713
DISU vs. IL7-0.3626851778633020.3799860746903380.750660473850981
DISU vs. SAHA-0.8500717728487890.02243930119271560.174410886165765
DMSO vs. AZA-0.06609168378094470.7007856313058161
DMSO vs. CD3-1.178914157095690.137426452342640.221378261820132
DMSO vs. DISU-0.5967392311305230.0955003229332380.561569786929451
DMSO vs. IL70.247445004974810.2935707507397690.780568412101396
DMSO vs. SAHA-0.2636241570291150.269999913135910.619544563165546
HIV vs. Mock in Activation-0.1216120012380080.9391848774663020.999983755607037
HIV vs. Mock in Latency-0.08177702119503580.6278071961539040.999834320637052
IL7 vs. CD3-0.9166489386541480.2499398040801890.381696318834924
SAHA vs. CD3-1.448333697326260.07280463190388720.13503579111281
SAHA vs. IL7-0.5009437590956920.0498294510732840.183740006232554
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.538309 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.773 0.907 0.699 0.479 0.678
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found