Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001727
UniProt IDP49736
Primary gene name(s)MCM2
Synonym gene name(s)BM28, CCNL1, CDCL1, KIAA0030
Protein nameDNA replication licensing factor MCM2
Protein functionActs as component of the MCM2-7 complex, MCM complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis. {ECO:0000269|PubMed:26196677, ECO:0000269|PubMed:8175912}.
Subcellular locationNucleus {ECO:0000269|PubMed:8175912}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P49736
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
cellular response to interleukin-4 [GO:0071353];
cochlea development [GO:0090102];
DNA replication [GO:0006260];
DNA replication initiation [GO:0006270];
DNA unwinding involved in DNA replication [GO:0006268];
G1/S transition of mitotic cell cycle [GO:0000082];
nucleosome assembly [GO:0006334]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
DNA binding [GO:0003677];
DNA helicase activity [GO:0003678];
DNA replication origin binding [GO:0003688];
metal ion binding [GO:0046872]
Gene Ontology
(Cellular Component)
Complete annatation
chromatin [GO:0000785];
cytoplasm [GO:0005737];
MCM complex [GO:0042555];
microtubule cytoskeleton [GO:0015630];
nuclear chromosome, telomeric region [GO:0000784];
nuclear origin of replication recognition complex [GO:0005664];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction110339
Phylogenetic treeP49736
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD33.024602251235362.29471725732289e-072.04047070810446e-06
AZA vs. DISU-0.3998484800821410.1181559570494110.672488840231307
AZA vs. IL71.19622719255050.000384365166072320.0399554217117799
AZA vs. SAHA-0.2709399827958520.2733314341989880.647931756426411
DISU vs. CD3-3.436512688842717.60809781841232e-091.21745718168522e-07
DISU vs. IL71.586763217818395.4488610332637e-060.000543646001621255
DISU vs. SAHA0.1309562810582670.6558624165033180.894349642029374
DMSO vs. AZA0.0170132173093610.9209006437405121
DMSO vs. CD3-3.018977838167722.35372844992199e-071.90332362308506e-06
DMSO vs. DISU0.4147295380766210.09275794043561070.558208626966296
DMSO vs. IL71.186883367811720.0004527354724896960.0326387771981195
DMSO vs. SAHA-0.2935164639309610.2186736290824650.562298287647471
HIV vs. Mock in Activation0.1135753733793060.9238147438279940.999983755607037
HIV vs. Mock in Latency0.04137215697804190.852884346025910.999834320637052
IL7 vs. CD3-1.82210644038560.003603300134511490.0127225446070489
SAHA vs. CD3-3.31936509441063.41927481883886e-084.06347844979147e-07
SAHA vs. IL7-1.469315841277844.85371475132945e-050.00109364568253423
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
1.9 0.000262212 1.2 0.079540676 1.3 0.22893669
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.416849 0.00132191
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
0.046 0.005

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.025 0.934 0.899 0.866 0.979
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4UUZ X-ray 2.9Å C=69-138.
5BNV X-ray 2.7Å C/F=61-130.
5BNX X-ray 2.3Å C=61-130.
5BO0 X-ray 2.9Å C=61-130.
5C3I X-ray 3.5Å D/H/L/P/T/X=63-124.
5JA4 X-ray 2.4Å C=61-130.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat upregulates 23364796

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03030 DNA replication - Homo sapiens (human)
hsa04110 Cell cycle - Homo sapiens (human)