Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001726
UniProt IDQ7L590
Primary gene name(s)MCM10
Synonym gene name(s)unknown
Protein nameProtein MCM10 homolog
Protein functionActs as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability; acts as a direct target of transcriptional repression by ZBTB38, PubMed:24726359. {ECO:0000269|PubMed:11095689, ECO:0000269|PubMed:15136575, ECO:0000269|PubMed:17699597, ECO:0000269|PubMed:19608746, ECO:0000269|PubMed:24726359}.
Subcellular locationNucleus {ECO:0000269|PubMed:11095689, ECO:0000269|PubMed:15136575}. Note=Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q7L590
Gene Ontology
(Biological Process)
Complete annatation
cell proliferation [GO:0008283];
cellular response to DNA damage stimulus [GO:0006974];
DNA replication [GO:0006260];
DNA replication initiation [GO:0006270];
G1/S transition of mitotic cell cycle [GO:0000082]
Gene Ontology
(Molecular Function)
Complete annatation
DNA replication origin binding [GO:0003688];
identical protein binding [GO:0042802];
metal ion binding [GO:0046872];
single-stranded DNA binding [GO:0003697]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
nucleolus [GO:0005730];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
replication fork protection complex [GO:0031298]
Protein-protein interaction120654
Phylogenetic treeQ7L590
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD33.31224357493410.02523682847443710.0563928063405064
AZA vs. DISU-0.2047783068035480.6110487916032320.957450739791279
AZA vs. IL71.308323440566160.02646142121568610.521376473181747
AZA vs. SAHA-0.7466098825841090.1275354369085460.454395220216194
DISU vs. CD3-3.5309195693640.01583291767840630.042391723105559
DISU vs. IL71.505219577407810.01276251469245450.136547132000533
DISU vs. SAHA-0.5422231512007780.2840733405606210.666449146262316
DMSO vs. AZA-0.1196641434698250.7435729064254421
DMSO vs. CD3-3.444550788595620.01950257733522730.0438909960494771
DMSO vs. DISU0.08243064330436250.8311951357071690.98111438763097
DMSO vs. IL71.435649090207210.01354893735487210.245788484506253
DMSO vs. SAHA-0.6353263485986830.1818410813505680.514669927676862
HIV vs. Mock in Activation-0.1011799301082680.9660822478662970.999983755607037
HIV vs. Mock in Latency-0.04247994613327820.9082575592752480.999834320637052
IL7 vs. CD3-1.994793481137710.1620152953668430.275440621144194
SAHA vs. CD3-4.092640782588270.00574903835233620.0160619221300902
SAHA vs. IL7-2.063085007727340.002081538499022820.0202563662584054
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
2.3 6.61E-05 1.3 0.140910692 1.4 0.13147913
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.647227 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr depletes 26032416
Vpr degrades 26032416
Vpr enhances 26032416

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
not found