Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001725
UniProt IDQ07820
Primary gene name(s)MCL1
Synonym gene name(s)BCL2L3
Protein nameInduced myeloid leukemia cell differentiation protein Mcl-1
Protein functionInvolved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. {ECO:0000269|PubMed:10766760, ECO:0000269|PubMed:16543145}.
Subcellular locationMembrane {ECO:0000305};
Single-pass membrane protein {ECO:0000305}. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Note=Cytoplasmic, associated with mitochondria.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q07820
Gene Ontology
(Biological Process)
Complete annatation
apoptotic mitochondrial changes [GO:0008637];
cell fate determination [GO:0001709];
cellular homeostasis [GO:0019725];
extrinsic apoptotic signaling pathway in absence of ligand [GO:0097192];
intrinsic apoptotic signaling pathway in response to DNA damage [GO:0008630];
multicellular organism development [GO:0007275];
negative regulation of anoikis [GO:2000811];
negative regulation of apoptotic process [GO:0043066];
negative regulation of autophagy [GO:0010507];
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [GO:2001240];
negative regulation of intrinsic apoptotic signaling pathway [GO:2001243];
positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway [GO:1903378];
regulation of response to DNA damage stimulus [GO:2001020];
response to cytokine [GO:0034097]
Gene Ontology
(Molecular Function)
Complete annatation
BH3 domain binding [GO:0051434];
protein channel activity [GO:0015266];
protein heterodimerization activity [GO:0046982];
protein homodimerization activity [GO:0042803]
Gene Ontology
(Cellular Component)
Complete annatation
Bcl-2 family protein complex [GO:0097136];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
integral component of membrane [GO:0016021];
membrane [GO:0016020];
mitochondrial matrix [GO:0005759];
mitochondrial outer membrane [GO:0005741];
mitochondrion [GO:0005739];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction110338
Phylogenetic treeQ07820
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.4467395476127620.1723145314350840.271722600731281
AZA vs. DISU0.05126137154539580.8389360281540310.986623420073108
AZA vs. IL7-0.1733540988521450.3654067111417660.999311006273513
AZA vs. SAHA-0.1650471498797640.4974752020439590.819791303145915
DISU vs. CD30.486441710970340.1796811451332390.293733172295678
DISU vs. IL7-0.2342733879968990.3512805318971860.732180363653542
DISU vs. SAHA-0.2149907737122160.459791870492580.798937533514497
DMSO vs. AZA-0.07340492383051770.6595356111889471
DMSO vs. CD30.3648667487603490.2534224013190050.359729433324302
DMSO vs. DISU-0.1257894506159390.6051428486324780.939289650701039
DMSO vs. IL7-0.09315380812654920.6028882340577870.912604767320708
DMSO vs. SAHA-0.09892918947482350.6738756017808650.897786781843686
HIV vs. Mock in Activation-0.02989659092259660.9616554699340680.999983755607037
HIV vs. Mock in Latency0.007047553652548770.9657724630996170.999834320637052
IL7 vs. CD30.2804295966523190.3822373949977650.51895732794103
SAHA vs. CD30.2576563012058130.4650815503380350.576954171806685
SAHA vs. IL70.005215228272308280.9828750776407110.993710617125574
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.214326 0.240819
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.097 1.256 0.89 0.796 0.926
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2KBW NMR - A=163-326.
2MHS NMR - A=171-327.
2NL9 X-ray 1.5Å A=209-327.
2NLA X-ray 2.8Å A=209-327.
2PQK X-ray 2.0Å A=172-327.
3D7V X-ray 2.0Å A=209-327.
3IO9 X-ray 2.4Å A=209-327.
3KJ0 X-ray 1.7Å A=172-327.
3KJ1 X-ray 1.9Å A=172-327.
3KJ2 X-ray 2.3Å A=172-327.
3KZ0 X-ray 2.3Å A/B=172-327.
3MK8 X-ray 2.3Å A=172-327# B=208-228.
3PK1 X-ray 2.4Å A/C=174-326.
3TWU X-ray 1.8Å B=73-88.
3WIX X-ray 1.9Å A/B/C/D=172-327.
3WIY X-ray 2.1Å A/B/C/D/E/F=172-327.
4BPI X-ray 1.9Å A=209-327.
4BPJ X-ray 1.6Å A=209-327.
4HW2 X-ray 2.8Å A/B/C/D/E/F=172-323.
4HW3 X-ray 2.4Å A/B/C/D/E/F/G/H/I/J/K/L=172-323.
4HW4 X-ray 1.5Å A/B=172-327.
4OQ5 X-ray 2.8Å A/B/C/D/E/F=174-326.
4OQ6 X-ray 1.8Å A/B=174-326.
4WGI X-ray 1.8Å A=173-321.
4WMR X-ray 1.7Å A=173-321.
4WMS X-ray 1.9Å A=174-321.
4WMT X-ray 2.3Å A=174-321.
4WMU X-ray 1.5Å A=174-321.
4WMV X-ray 2.4Å A=174-321.
4WMW X-ray 1.9Å A=174-321.
4WMX X-ray 2.0Å A=174-321.
4ZBF X-ray 2.2Å A/B/C/D/E/F/G/H/I/J/K/L=172-327.
4ZBI X-ray 2.5Å A/B/C/D/E/F/G/H/I/J/K/L=172-327.
5C3F X-ray 1.4Å A=173-327.
5C6H X-ray 2.0Å A/C/E/G/I/K/M/O/Q/S/U/W=171-327.
5FC4 X-ray 1.5Å A=172-320.
5FDO X-ray 2.8Å A/B/C/D=172-320.
5FDR X-ray 2.6Å A/B/C/D=172-327.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr interacts with 22403404
Envelope surface glycoprotein gp120 upregulates 17907802
retropepsin interacts with 22190034

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04210 Apoptosis - Homo sapiens (human)
hsa04630 Jak-STAT signaling pathway - Homo sapiens (human)
hsa05206 MicroRNAs in cancer - Homo sapiens (human)