Protein Information (annotations from UniProt)
| Database ID | HIV0001725 |
| UniProt ID | Q07820 |
| Primary gene name(s) | MCL1 |
| Synonym gene name(s) | BCL2L3 |
| Protein name | Induced myeloid leukemia cell differentiation protein Mcl-1 |
| Protein function | Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. {ECO:0000269|PubMed:10766760, ECO:0000269|PubMed:16543145}. |
| Subcellular location | Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Note=Cytoplasmic, associated with mitochondria. |
| ECO code | Click here for more information. |
| Amino acid sequence FASTA format: Q07820 |
|
| Gene Ontology (Biological Process) Complete annatation | apoptotic mitochondrial changes [GO:0008637]; cell fate determination [GO:0001709]; cellular homeostasis [GO:0019725]; extrinsic apoptotic signaling pathway in absence of ligand [GO:0097192]; intrinsic apoptotic signaling pathway in response to DNA damage [GO:0008630]; multicellular organism development [GO:0007275]; negative regulation of anoikis [GO:2000811]; negative regulation of apoptotic process [GO:0043066]; negative regulation of autophagy [GO:0010507]; negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [GO:2001240]; negative regulation of intrinsic apoptotic signaling pathway [GO:2001243]; positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway [GO:1903378]; regulation of response to DNA damage stimulus [GO:2001020]; response to cytokine [GO:0034097] |
| Gene Ontology (Molecular Function) Complete annatation | BH3 domain binding [GO:0051434]; protein channel activity [GO:0015266]; protein heterodimerization activity [GO:0046982]; protein homodimerization activity [GO:0042803] |
| Gene Ontology (Cellular Component) Complete annatation | Bcl-2 family protein complex [GO:0097136]; cytoplasm [GO:0005737]; cytosol [GO:0005829]; integral component of membrane [GO:0016021]; membrane [GO:0016020]; mitochondrial matrix [GO:0005759]; mitochondrial outer membrane [GO:0005741]; mitochondrion [GO:0005739]; nucleoplasm [GO:0005654]; nucleus [GO:0005634] |
| Protein-protein interaction | 110338 |
| Phylogenetic tree | Q07820 |
| HIV replication factor status |
Zhou et al., Cell. Host. Microbe., 2008 unknown Brass et al., Science, 2008 unknown Smith et al., J. Immunol, 2010 unknown |
| Interferon-stimulated gene status |
Lu et al., J. Virol., 2011 Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown; Schoggins JW and Rice CM, Curr. Opin. Virol., 2011 Targeted viruses: unknown Viral life cycle: unknown Mechanism related to antiviral activity: unknown |
| Anti-viral restriction factor |
Liu et al., Retrovirology, 2011 unknown (Triplicates) |
Gene Expression Profile top
Up-regulated; 
Down-regulated
For brief introduction to each study, please go to the help page.
Gene expression during HIV latency
| (1). Mohammadi et al., PLoS Pathog., 2014 Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model DMSO: Dimethyl suloxyde (negative control) - 0.0033% final SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies IL7: Interleukin-7 based stimulation DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM |
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| Experimental Condition | Log2 Fold Change | P value | Adjusted P value |
| AZA vs. CD3 | -0.446739547612762 | 0.172314531435084 | 0.271722600731281 |
| AZA vs. DISU | 0.0512613715453958 | 0.838936028154031 | 0.986623420073108 |
| AZA vs. IL7 | -0.173354098852145 | 0.365406711141766 | 0.999311006273513 |
| AZA vs. SAHA | -0.165047149879764 | 0.497475202043959 | 0.819791303145915 |
| DISU vs. CD3 | 0.48644171097034 | 0.179681145133239 | 0.293733172295678 |
| DISU vs. IL7 | -0.234273387996899 | 0.351280531897186 | 0.732180363653542 |
| DISU vs. SAHA | -0.214990773712216 | 0.45979187049258 | 0.798937533514497 |
| DMSO vs. AZA | -0.0734049238305177 | 0.659535611188947 | 1 |
| DMSO vs. CD3 | 0.364866748760349 | 0.253422401319005 | 0.359729433324302 |
| DMSO vs. DISU | -0.125789450615939 | 0.605142848632478 | 0.939289650701039 |
| DMSO vs. IL7 | -0.0931538081265492 | 0.602888234057787 | 0.912604767320708 |
| DMSO vs. SAHA | -0.0989291894748235 | 0.673875601780865 | 0.897786781843686 |
| HIV vs. Mock in Activation | -0.0298965909225966 | 0.961655469934068 | 0.999983755607037 |
| HIV vs. Mock in Latency | 0.00704755365254877 | 0.965772463099617 | 0.999834320637052 |
| IL7 vs. CD3 | 0.280429596652319 | 0.382237394997765 | 0.51895732794103 |
| SAHA vs. CD3 | 0.257656301205813 | 0.465081550338035 | 0.576954171806685 |
| SAHA vs. IL7 | 0.00521522827230828 | 0.982875077640711 | 0.993710617125574 |
| (2). Iglesias-Ussel et al., J. Virol., 2013 Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model |
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| Log2 Fold Change | P Value | ||
| unknown | unknown | ||
Gene expression during HIV infection and replication
| (1). Imbeault et al., PloS Pathog., 2012 Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells |
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| Experiment type | Log2 Fold Change | P Value | Adjusted P Value | ||
| Infected vs. Mock | unknown | unknown | unknown | ||
| Infected vs. Bystander | unknown | unknown | unknown | ||
| (2). Lefebvre et al., J. Virol., 2011 Transcriptome analysis of T-cell line (Sup T1) |
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| Log2 Fold Change | unknown | ||||
| (3). Li et al., J. Immunol., 2013 Lymphatic tissue |
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| Acute Fold Change | Acute P Value | Asymt Fold Change | Asypt P Value | AIDS Fold Change | AIDS P Value |
| unknown | unknown | unknown | unknown | unknown | unknown |
| (4). Chang et al., MBio., 2011 Transcriptome analysis of T-cell line (Sup T1) Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation |
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| Up-regulated (True) | FALSE | ||||
| (5). Sherrill-Mix et al., BMC Retrovirol., 2015 Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based |
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| Test Status | Log2 Fold Change | P Value | |||
| OK | -0.214326 | 0.240819 | |||
| (6). Rotger et al., PLoS Pathog., 2010 Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient (Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach) |
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| Log2 Fold Change | P Value | ||||
| unknown | unknown | ||||
Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV
| (1). Greenwood et al., Elife, 2016 Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset |
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| 6 h | 24 h | 48 h | 72 h | RTi | ||
| 1.097 | 1.256 | 0.89 | 0.796 | 0.926 | ||
| (2). Navare et al., Virology, 2012 SUP-T1 cell line |
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| FC-4hpi | P-value | FC-8hpi | P-value | FC-20hpi | P-value | Category |
| unknown | unknown | unknown | unknown | unknown | unknown | unknown |
| (3). Hyrcza et al., J. Virolo., 2007 Primary human CD4+ and CD8+ T Cells |
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| Affymetrix Prob ID | Fold Change | In CD8? | Category | |||
| unknown | unknown | unknown | unknown | |||
Protein Overview top
Drug-protein Interaction (annotations from DrugBank) top
Protein Secondary Structure (annotations from PDB) top
| PDB Accession | Method | Resolution | Chain | Structure Preview |
|---|---|---|---|---|
| 2KBW | NMR | - | A=163-326. |  |
| 2MHS | NMR | - | A=171-327. |  |
| 2NL9 | X-ray | 1.5Å | A=209-327. |  |
| 2NLA | X-ray | 2.8Å | A=209-327. |  |
| 2PQK | X-ray | 2.0Å | A=172-327. |  |
| 3D7V | X-ray | 2.0Å | A=209-327. |  |
| 3IO9 | X-ray | 2.4Å | A=209-327. |  |
| 3KJ0 | X-ray | 1.7Å | A=172-327. |  |
| 3KJ1 | X-ray | 1.9Å | A=172-327. |  |
| 3KJ2 | X-ray | 2.3Å | A=172-327. |  |
| 3KZ0 | X-ray | 2.3Å | A/B=172-327. |  |
| 3MK8 | X-ray | 2.3Å | A=172-327# B=208-228. |  |
| 3PK1 | X-ray | 2.4Å | A/C=174-326. |  |
| 3TWU | X-ray | 1.8Å | B=73-88. |  |
| 3WIX | X-ray | 1.9Å | A/B/C/D=172-327. |  |
| 3WIY | X-ray | 2.1Å | A/B/C/D/E/F=172-327. |  |
| 4BPI | X-ray | 1.9Å | A=209-327. |  |
| 4BPJ | X-ray | 1.6Å | A=209-327. |  |
| 4HW2 | X-ray | 2.8Å | A/B/C/D/E/F=172-323. |  |
| 4HW3 | X-ray | 2.4Å | A/B/C/D/E/F/G/H/I/J/K/L=172-323. |  |
| 4HW4 | X-ray | 1.5Å | A/B=172-327. |  |
| 4OQ5 | X-ray | 2.8Å | A/B/C/D/E/F=174-326. |  |
| 4OQ6 | X-ray | 1.8Å | A/B=174-326. |  |
| 4WGI | X-ray | 1.8Å | A=173-321. |  |
| 4WMR | X-ray | 1.7Å | A=173-321. |  |
| 4WMS | X-ray | 1.9Å | A=174-321. |  |
| 4WMT | X-ray | 2.3Å | A=174-321. |  |
| 4WMU | X-ray | 1.5Å | A=174-321. |  |
| 4WMV | X-ray | 2.4Å | A=174-321. |  |
| 4WMW | X-ray | 1.9Å | A=174-321. |  |
| 4WMX | X-ray | 2.0Å | A=174-321. |  |
| 4ZBF | X-ray | 2.2Å | A/B/C/D/E/F/G/H/I/J/K/L=172-327. |  |
| 4ZBI | X-ray | 2.5Å | A/B/C/D/E/F/G/H/I/J/K/L=172-327. |  |
| 5C3F | X-ray | 1.4Å | A=173-327. |  |
| 5C6H | X-ray | 2.0Å | A/C/E/G/I/K/M/O/Q/S/U/W=171-327. |  |
| 5FC4 | X-ray | 1.5Å | A=172-320. |  |
| 5FDO | X-ray | 2.8Å | A/B/C/D=172-320. |  |
| 5FDR | X-ray | 2.6Å | A/B/C/D=172-327. |  |