Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001716
UniProt IDQ9NZL9
Primary gene name(s)MAT2B
Synonym gene name(s)TGR
Protein nameMethionine adenosyltransferase 2 subunit beta
Protein functionRegulatory subunit of S-adenosylmethionine synthetase 2, an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates MAT2A catalytic activity by changing its kinetic properties, increasing its affinity for L-methionine, PubMed:10644686, PubMed:23189196, PubMed:25075345. Can bind NADP, in vitro, PubMed:23189196, PubMed:23425511. {ECO:0000269|PubMed:10644686, ECO:0000269|PubMed:23189196, ECO:0000269|PubMed:23425511, ECO:0000269|PubMed:25075345}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NZL9
Gene Ontology
(Biological Process)
Complete annatation
methylation [GO:0032259];
one-carbon metabolic process [GO:0006730];
S-adenosylmethionine biosynthetic process [GO:0006556]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
methionine adenosyltransferase regulator activity [GO:0048270]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
intracellular [GO:0005622];
methionine adenosyltransferase complex [GO:0048269];
mitochondrion [GO:0005739];
nucleus [GO:0005634]
Protein-protein interaction118166
Phylogenetic treeQ9NZL9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.09312061894085530.7764023426817360.845760054126853
AZA vs. DISU0.08110932384451680.7479858925729220.976008954784031
AZA vs. IL7-0.157775467305570.4110022353331450.999311006273513
AZA vs. SAHA-0.1572571046325160.5184253686371180.832595781678662
DISU vs. CD30.1620355665687390.6549908154168880.754611093824073
DISU vs. IL7-0.2484249942996940.3234562055900360.7037098283889
DISU vs. SAHA-0.2369591183934310.415580866212850.771176669144787
DMSO vs. AZA-0.05662697814220870.7345531646810531
DMSO vs. CD30.02621145847761840.9347706783474990.95460413973055
DMSO vs. DISU-0.1392949626271530.5673631743649440.928202810947288
DMSO vs. IL7-0.0942160823701560.5996218479151530.911970258876874
DMSO vs. SAHA-0.1073021447878020.6483708110529960.886888304039713
HIV vs. Mock in Activation-0.1359604959226160.8273424408217140.999983755607037
HIV vs. Mock in Latency0.1967785204518650.2317679406981490.999834320637052
IL7 vs. CD3-0.05734857530603460.8581864009912410.909451200221016
SAHA vs. CD3-0.08794400285570510.8038784110007570.860829080777475
SAHA vs. IL7-0.002619218433903830.9914108715124310.996359612269625
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.33461 0.0167513
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.97 1.166 1.29 1.146 1.196
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00134 L-Methionine approved, nutraceutical unknown substrate

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2YDX X-ray 2.8Å A/B/C/D/E=28-334.
2YDY X-ray 2.2Å A=28-334.
4KTT X-ray 2.5Å E/F=22-334.
4KTV X-ray 3.3Å E/F=22-334.
4NDN X-ray 2.3Å E/F=22-334.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00270 Cysteine and methionine metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01230 Biosynthesis of amino acids - Homo sapiens (human)