Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0001715
UniProt IDP31153
Primary gene name(s)MAT2A
Synonym gene name(s)AMS2, MATA2
Protein nameS-adenosylmethionine synthase isoform type-2
Protein functionCatalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine, AdoMet and triphosphate, and subsequent hydrolysis of the triphosphate. {ECO:0000269|PubMed:10644686, ECO:0000269|PubMed:23189196, ECO:0000269|PubMed:25075345}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P31153
Gene Ontology
(Biological Process)
Complete annatation
methylation [GO:0032259];
one-carbon metabolic process [GO:0006730];
protein heterooligomerization [GO:0051291];
protein hexamerization [GO:0034214];
S-adenosylmethionine biosynthetic process [GO:0006556]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
metal ion binding [GO:0046872];
methionine adenosyltransferase activity [GO:0004478]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
methionine adenosyltransferase complex [GO:0048269]
Protein-protein interaction110314
Phylogenetic treeP31153
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.264389154208290.000139381463238220.000648381917878532
AZA vs. DISU-0.2159374085993730.3934581285286370.902399430427972
AZA vs. IL70.4838356190787260.01180550479845090.358758818199698
AZA vs. SAHA-0.2851145922205940.2423475763037240.614267554571613
DISU vs. CD3-1.492945808122635.62263002132912e-050.000342267752660105
DISU vs. IL70.6906886208334390.006296477077146870.0866843761990767
DISU vs. SAHA-0.0676058837230430.8171766572678040.953084396891188
DMSO vs. AZA0.08892937524396250.5943823804575181
DMSO vs. CD3-1.186861069178250.0002450944309684290.000997392653306154
DMSO vs. DISU0.3031605119372910.2141711853475320.726625098239347
DMSO vs. IL70.4021532021722680.02509923832411510.322017887434923
DMSO vs. SAHA-0.3803619802545310.1068485361112090.387107064971989
HIV vs. Mock in Activation0.09533841227000950.8780282462753970.999983755607037
HIV vs. Mock in Latency-0.03121717882867730.8494278010450470.999834320637052
IL7 vs. CD3-0.7733476031675560.01652430563898520.0450317951358568
SAHA vs. CD3-1.573506467430141.30536369993273e-057.90986367430084e-05
SAHA vs. IL7-0.7720328473376260.001606380545576160.0167774882208048
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
-1.2 0.173406426 -1.4 0.009469255 -1.7 0.015453282
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) FALSE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.278641 0.0769962
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.963 0.91 0.748 0.756 0.983
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00118 S-Adenosylmethionine approved, nutraceutical unknown cofactor
DB00134 L-Methionine approved, nutraceutical unknown substrate

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2P02 X-ray 1.2Å A=1-395.
4KTT X-ray 2.5Å A/B/C/D=1-395.
4KTV X-ray 3.3Å A/B/C/D=1-395.
4NDN X-ray 2.3Å A/B/C/D=1-395.
5A19 X-ray 2.3Å A=1-395.
5A1G X-ray 1.8Å A=1-395.
5A1I X-ray 1.0Å A=1-395.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18854154

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00270 Cysteine and methionine metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01230 Biosynthesis of amino acids - Homo sapiens (human)